The antioxidant activity of alpha-tocopherol-bovine serum albumin complex in micellar and liposome autoxidations.
The antioxidant activity of alpha-tocopherol-bovine serum albumin complex in micellar and liposome autoxidations.
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α-生育酚-牛血清白蛋白复合物在胶束和脂质体自氧化中的抗氧化活性。
DOI：
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发表时间：
1985
影响因子：
4.8
通讯作者：
D. Kong
中科院分区：
文献类型：
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作者：
L. Barclay;A. M. Bailey;D. Kong
比较了α-生育酚与牛血清白蛋白（α-TX BSA）复合的水溶性形式与胶束α-生育酚和6-羟基-2，5，7，8-四甲基苯并二氢吡喃-2-羧酸（Trolox）水溶液抑制亚油酸在十二烷基硫酸钠胶束中自氧化的抗氧化活性。过氧化氢自由基捕获能力的α-TX BSA相比有利的α-生育酚和Trolox，和所有三个可以用于定量测量的敏感性的胶束基板进行自氧化：氧化性，在胶束相引发的反应由二叔丁基次硝酸盐（DBHN）或在水相中由偶氮双脒基丙烷盐酸盐（ABAP）。α-生育酚和Trolox也是抑制DBHN或ABAP引发的二亚油酰磷脂酰胆碱（DLPC）脂质体的自氧化的有效抗氧化剂，所述脂质体制备为通过31 P NMR光谱表征的多层或单层双层。DLPC脂质体的氧化性由水溶性和脂溶性引发剂与抗氧化剂α-生育酚和Trolox的各种组合决定。相比之下，当在脂质相（DBHN）或水相（ABAP）中引发自氧化后加入α-TX BSA时，α-TX BSA不能有效地捕获过氧自由基。如果在引发前将α-TX BSA与DLPC混合数小时，则α-TX BSA的自由基捕获能力变得明显。该结果解释为α-生育酚在表现出抗氧化活性之前从结合的α-TX BSA形式扩散到脂质体。
A comparison is made of the antioxidant activity of a water-soluble form of alpha-tocopherol complexed with bovine serum albumin (alpha-T X BSA) with that of micellar alpha-tocopherol and aqueous 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylate (Trolox) to inhibit autoxidation of linoleic acid in sodium dodecyl sulfate micelles. The peroxyl radical trapping ability of alpha-T X BSA compares favorably with that of alpha-tocopherol and Trolox, and all three can be used in quantitative measurements of the susceptibility of the micellar substrate to undergo autoxidation: the oxidizability, for reactions initiated in the micellar phase by di-tertbutylhyponitrite (DBHN) or in the aqueous phase by azobisamidinopropane hydrochloride (ABAP). alpha-Tocopherol and Trolox are also effective antioxidants to inhibit DBHN- or ABAP-initiated autoxidations of dilinoleoylphosphatidylcholine (DLPC) liposomes prepared as multilamellar or unilamellar bilayers characterized by 31P NMR spectra. The oxidizability of DLPC liposomes is determined by various combinations of water-soluble and lipid-soluble initiators and the antioxidants, alpha-tocopherol and Trolox. In contrast, alpha-T X BSA does not effectively trap peroxyl radicals when it is added after initiation of autoxidation in the lipid phase (DBHN) or in the aqueous phase (ABAP). The radical trapping ability of alpha-T X BSA becomes evident if it is mixed with the DLPC for some hours before initiation. This result is interpreted in terms of diffusion of alpha-tocopherol from the bound alpha-T X BSA form to the liposome before it exhibits antioxidant activity.