Streptomyces sanglieri which colonised and enhanced the growth of Elaeis guineensis Jacq. seedlings was antagonistic to Ganoderma boninense in in vitro studies
Streptomyces sanglieri which colonised and enhanced the growth of Elaeis guineensis Jacq. seedlings was antagonistic to Ganoderma boninense in in vitro studies
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DOI：
10.1007/s10295-015-1724-4
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发表时间：
2016-04-01
影响因子：
3.4
通讯作者：
Vikineswary, S.
中科院分区：
文献类型：
--
作者：
Azura, A. B. Nur;Yusoff, M.;Vikineswary, S.
放线菌菌株AUM 00500与桑氏链霉菌NBRC 100784（T）的相似性为99.5%，并对油棕茎基腐病的病原菌---博宁灵芝（Ganoderma boninense）进行了拮抗活性评价。该菌株对G.离体培养和扫描电镜分析表明，该真菌的抑制模式多种多样。单培养物乙酸乙酯提取物和交叉培养物抑菌圈HPLC测定表明，菌株AUM 00500产生两种不同的戊二酰亚胺类抗生素，即放线菌酮和放线菌酚。在温室试验中，用S.当与对照相比时，10（9）cfu/ml的桑列里菌株AUM 00500显示出油棕幼苗生长的显著增加（P < 0.05）。桑氏链霉菌菌株AUM 00500成功地定殖于处理过的油棕幼苗的根的表皮表面，并且从铺在淀粉酪蛋白琼脂上的根片段中回收。
Actinomycete strain AUM 00500 was 99.5 % similar to Streptomyces sanglieri NBRC 100784(T) and was evaluated for antagonistic activity towards Ganoderma boninense, the causative fungus of basal stem rot of oil palm. The strain showed strong antifungal activity towards G. boninense in in vitro and SEM analysis showed various modes of inhibition of the fungus. Ethyl acetate extracts of single culture and inhibition zone of cross-plug culture by HPLC indicated that strain AUM 00500 produced two different antibiotics of the glutarimide group namely cycloheximide and actiphenol. In greenhouse trials, oil palm seed treated with spores of S. sanglieri strain AUM 00500 at 10(9) cfu/ml showed significant (P < 0.05) increase in oil palm seedlings growth when compared to the control. Streptomyces sanglieri strain AUM 00500 successfully colonised the epidermal surface of the roots of treated oil palm seedlings and it was recovered from root fragments plated on starch casein agar.