Human monoclonal antibody as prophylaxis for SARS coronavirus infection in ferrets.
Human monoclonal antibody as prophylaxis for SARS coronavirus infection in ferrets.
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DOI：
10.1016/s0140-6736(04)16506-9
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发表时间：
2004-06-26
期刊：
影响因子：
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通讯作者：
Osterhaus AD
中科院分区：
文献类型：
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作者：
ter Meulen J;Bakker AB;van den Brink EN;Weverling GJ;Martina BE;Haagmans BL;Kuiken T;de Kruif J;Preiser W;Spaan W;Gelderblom HR;Goudsmit J;Osterhaus AD
SARS冠状病毒继续在中国造成散发的严重急性呼吸综合征（SARS）病例。目前尚无针对SARS冠状病毒引起的疾病的主动或被动免疫预防措施。我们研究了预防SARS冠状病毒感染的中和人单克隆抗体在雪貂，这可以很容易地被病毒感染。预防性给予10 mg/kg的单克隆抗体可使感染雪貂肺中SARS冠状病毒的复制减少3.3 log（95%Cl 2·6- 4.0 log; p<0.001），完全阻止SARS冠状病毒诱导的肉眼可见肺病理学的发展（p= 0.013），并消除咽分泌物中的病毒脱落。该动物模型产生的数据表明，给予人单克隆抗体可能为控制人SARS冠状病毒感染提供可行且有效的预防措施。
SARS coronavirus continues to cause sporadic cases of severe acute respiratory syndrome (SARS) in China. No active or passive immunoprophylaxis for disease induced by SARS coronavirus is available. We investigated prophylaxis of SARS coronavirus infection with a neutralising human monoclonal antibody in ferrets, which can be readily infected with the virus. Prophylactic administration of the monoclonal antibody at 10 mg/kg reduced replication of SARS coronavirus in the lungs of infected ferrets by 3·3 logs (95% Cl 2·6–4·0 logs; p<0·001), completely prevented the development of SARS coronavirus-induced macroscopic lung pathology (p=0·013), and abolished shedding of virus in pharyngeal secretions. The data generated in this animal model show that administration of a human monoclonal antibody might offer a feasible and effective prophylaxis for the control of human SARS coronavirus infection.