Pattern of superoxide dismutase enzymatic activity and RNA changes in rat heart ventricles after myocardial infarction.
Pattern of superoxide dismutase enzymatic activity and RNA changes in rat heart ventricles after myocardial infarction.
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发表时间：
1997-08
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通讯作者：
M. Assem;J. Teyssier;M. Benderitter;J. Terrand;A. Laubriet;A. Javouhey;M. David;L. Rochette
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作者：
M. Assem;J. Teyssier;M. Benderitter;J. Terrand;A. Laubriet;A. Javouhey;M. David;L. Rochette
采用多重逆转录聚合酶链反应法测定左冠状动脉闭塞致心肌梗死后大鼠左、右心室锰超氧化物歧化酶（MnSOD）和CuZnSOD mrna的表达。这些数据与酶活性的变化进行了比较。在左心室，Mn-SOD RNA在6小时显著升高，在12小时达到峰值（490 +/- 38任意单位），然后逐渐降低（48小时127 +/- 21任意单位）。相比之下，右心室转录本的稳定积累可达48小时。在两个心室中，MnSOD mRNA和蛋白含量的变化与酶活性的比例变化无关。48小时内两心室CuZnSOD系统未见特征性改变。这些结果表明，梗死选择性地激活了两心室活心肌的MnSOD基因。他们认为MnSOD可能参与了肌细胞对超负荷应激的适应性反应。
A multiplex reverse transcription polymerase chain reaction assay was designed to measure manganese superoxide dismutase (MnSOD) and CuZnSOD mRNAs in the left and right ventricles of rat hearts after myocardial infarction induced by occlusion of the left coronary artery. These data were compared with changes in enzymatic activities. In the left ventricle, Mn-SOD RNA increased significantly at 6 hours, peaked at 12 hours (490 +/- 38 arbitrary units), and progressively decreased (127 +/- 21 arbitrary units at 48 hours). In contrast, there was a steady accumulation of transcripts in the right ventricle up to 48 hours. In both ventricles, the changes in the MnSOD mRNA and protein content were not associated with proportional variations in enzymatic activity. There was no characteristic alteration of the CuZnSOD system in either ventricle over the 48-hour period. These results demonstrate that infarction selectively activates the MnSOD gene in the viable myocardium of both ventricles. They suggest that MnSOD may be involved in the adaptive response of myocytes to the overloading stress.