SEQUENCE-DEPENDENT HYDROLYSIS OF RNA USING MODIFIED OLIGONUCLEOTIDE SPLINTS AND RNASE H
SEQUENCE-DEPENDENT HYDROLYSIS OF RNA USING MODIFIED OLIGONUCLEOTIDE SPLINTS AND RNASE H
复制标题
DOI：
10.1016/0014-5793(87)80171-0
复制
发表时间：
1987-05-11
期刊：
影响因子：
3.5
通讯作者：
OHTSUKA, E
中科院分区：
文献类型：
--
作者：
INOUE, H;HAYASE, Y;OHTSUKA, E
我们发现，在含有互补脱氧核糖核苷和2′-O-甲基核苷的嵌合寡核苷酸存在下，一个九核糖核苷酸[5′-32 pACUUACCUG]在用RNase H处理后被特异性切割。当3′m（UG）d（AATG）m（GAC）5′用作杂交链时，*pACUUACCUG在C6和C7之间被切割，产生 *pACUUAC。在3′m（UGAA）d（TGGA）m（C）5′存在下，九核糖核苷酸主要在U8和C9之间水解，得到 *pACUUACCU。该方法在RNA工程领域具有广泛的应用前景。
We found that, in the presence of chimeric oligonucleotides containing complementary deoxyribo‐ and 2′‐O‐methylnucleosides, a nonaribonucleotide, [5′‐32pACUUACCUG, was cleaved specifically upon treatment with RNase H. When 3′m(UG)d(AATG)m(GAC)5′ was used as a hybridization strand, *pACUUACCUG was cleaved between C6 and C7 to yield *pACUUAC. In the presence of 3′m(UGAA)d(TGGA)m(C)5′, the nonaribonucleotide was hydrolyzed, mainly between U8 and C9, to give *pACUUACCU. This method will have a variety of applications in the field of RNA engineering.