Lipidomics of glycosphingolipids.
Lipidomics of glycosphingolipids.
复制标题
鞘糖脂的脂质组学。
DOI：
10.3390/metabo2010134
复制
发表时间：
2012-02-02
期刊：
影响因子：
4.1
通讯作者：
Kolter T
中科院分区：
文献类型：
--
作者：
Farwanah H;Kolter T
鞘糖脂（GSL）含有一个或多个连接到鞘脂部分的糖，通常连接到神经酰胺，但在极少数情况下也连接到鞘氨醇碱。大的结构异质性是由碳水化合物残基的数量、身份、连接和端基异构体构型的差异以及疏水部分内的结构差异造成的。GSL形成复杂的细胞类型特异性模式，其随物种、细胞分化状态、病毒转化、个体发育和肿瘤发生而变化。虽然GSL结构可以被分配到只有几个系列与一个共同的碳水化合物的核心，其结构的多样性和复杂的模式是他们的解析和定量质谱技术的挑战。本文对脂质组学在GSL测定中的应用作一综述。这包括分析程序和仪器以及GSL分子种类与人类疾病的最新相关性。讨论了复杂GSL的结构复杂性和缺乏标准物质等困难。
Glycosphingolipids (GSLs) contain one or more sugars that are attached to a sphingolipid moiety, usually to a ceramide, but in rare cases also to a sphingoid base. A large structural heterogeneity results from differences in number, identity, linkage, and anomeric configuration of the carbohydrate residues, and also from structural differences within the hydrophobic part. GSLs form complex cell-type specific patterns, which change with the species, the cellular differentiation state, viral transformation, ontogenesis, and oncogenesis. Although GSL structures can be assigned to only a few series with a common carbohydrate core, their structural variety and the complex pattern are challenges for their elucidation and quantification by mass spectrometric techniques. We present a general overview of the application of lipidomics for GSL determination. This includes analytical procedures and instrumentation together with recent correlations of GSL molecular species with human diseases. Difficulties such as the structural complexity and the lack of standard substances for complex GSLs are discussed.