Correlation between Putative Inhibitory Molecules at the Dorsal Root Entry Zone and Failure of Dorsal Root Axonal Regeneration
Correlation between Putative Inhibitory Molecules at the Dorsal Root Entry Zone and Failure of Dorsal Root Axonal Regeneration
复制标题
背根进入区假定的抑制分子与背根轴突再生失败之间的相关性
DOI：
--
复制
发表时间：
2001
影响因子：
3.5
通讯作者：
P. Anderson
中科院分区：
文献类型：
--
作者：
Yi Zhang;K. Tohyama;J. Winterbottom;N.S.K. Haque;Melitta Schachner;A. Lieberman;P. Anderson
参与阻止再生的背根轴突在背根进入区（DREZ）进入脊髓的分子机制尚不清楚。我们使用免疫组织化学，原位杂交和电子显微镜研究轴突再生后，背根切断术在成年大鼠和它的关系，细胞的变化和假定的生长抑制分子在这一地区的分布。星形胶质细胞的过程，结束作为灯泡形的扩张，增长到700 microm到基膜管受伤的根，再生轴突也存在。其中一些轴突接近或到达DREZ，但没有进一步生长;其他轴突转向神经节，表明DREZ内或附近存在排斥性线索。生腱蛋白-C的mRNA和蛋白质和CSPG存根免疫反应强烈上调后，根，但只有弱表达的DREZ。腱生蛋白-R的免疫反应仅限于中枢神经系统组织，并不受神经根切断术。大，圆形的GFAP阴性，NG 2免疫反应细胞，其中一些是OX 42阳性，被发现在DREZ神经根切断术后。星形胶质细胞突起突出到根部是tenascin-R和NG 2阴性。因此，只有NG 2表达细胞和腱生蛋白-R被适当地定位以抑制通过DREZ的再生。
The molecular mechanisms involved in preventing regenerating dorsal root axons from entering the spinal cord at the dorsal root entry zone (DREZ) are obscure. We used immunohistochemistry, in situ hybridization, and electron microscopy to study axonal regeneration after dorsal rhizotomy in adult rats and its relationship to cellular changes and the distribution of putative growth inhibitory molecules in this region. Astrocyte processes, ending as bulb-shaped expansions, grew up to 700 microm into the basal lamina tubes of injured roots, where regenerating axons were also present. Some of these axons approached or reached the DREZ but grew no further; others turned back toward the ganglion, suggesting the presence of repulsive cues in or near the DREZ. Tenascin-C mRNA and protein and CSPG stub immunoreactivity were strongly upregulated in the roots after rhizotomy, but were only weakly expressed in the DREZ. Tenascin-R immunoreactivity was confined to CNS tissue, and unaffected by rhizotomy. Large, rounded GFAP-negative, NG2-immunoreactive cells, a few of which were OX42 positive, were found in the DREZ following rhizotomy. Astrocyte processes projecting into the roots were tenascin-R and NG2 negative. Hence, only NG2-expressing cells and tenascin-R were appropriately situated to inhibit regeneration through the DREZ.
登录
查看更多内容
正常和病理状态下的胶质祖细胞。
DOI：
10.2302/kjm.47.205
发表时间：
1998
期刊：
The Keio journal of medicine
影响因子：
--
作者：
Nishiyama,A
通讯作者：
Nishiyama,A
NG2 硫酸软骨素蛋白多糖：一种与未成熟细胞相关的多功能蛋白多糖。
DOI：
--
发表时间：
1996
期刊：
Perspectives on developmental neurobiology
影响因子：
--
作者：
Levine,JM;Nishiyama,A
通讯作者：
Nishiyama,A
DOI：
10.1073/pnas.94.19.10116
发表时间：
1997-09-16
影响因子：
11.1
作者：
Aspberg, A;Miura, R;Yamaguchi, Y
通讯作者：
Yamaguchi, Y
DOI：
10.1002/(sici)1097-4695(199801)34:1
发表时间：
1998-01-01
期刊：
JOURNAL OF NEUROBIOLOGY
影响因子：
--
作者：
Zuo, J;Hernandez, YJ;Muir, D
通讯作者：
Muir, D