DNA Methylation Analysis of the Gene CDKN2B in Gallus gallus (Chicken)
DNA Methylation Analysis of the Gene CDKN2B in Gallus gallus (Chicken)
复制标题
DOI：
10.3409/fb61_3-4.165
复制
发表时间：
2013-01-01
影响因子：
0.7
通讯作者：
Jocek, Grzegorz
中科院分区：
文献类型：
--
作者：
Gryzinska, Magdalena;Andraszek, Katarzyna;Jocek, Grzegorz
甲基化是DNA的表观遗传修饰，影响基因表达而不改变核苷酸的结构。它在生物体的胚胎和胚胎后发育中起着至关重要的作用。甲基化水平具有组织和物种特异性，并随年龄而变化。采用甲基化特异性PCR（methylation-specific PCR，MSP）方法检测了波尔巴鸡bar位点CDKN 2B基因在胚胎发育第6天和第18天的甲基化。在胚胎发育的第6天和第18天，CDKN 2B基因启动子区未检测到甲基化。作为黑素细胞中负责黑色素活性的五个基因之一，并且高度活跃，它可以促进这种色素的产生。本研究拓宽了对鸡表观基因组和鸟类自性别鉴定机制的认识。
Methylation is an epigenetic modification of DNA affecting gene expression without changing the structure of nucleotides. It plays a crucial role in the embryonic and post-embryonic development of living organisms. Methylation level is tissue and species-specific and changes with age. The study was aimed at identifying the methylation of the CDKN2B gene situated at locus bar in Polbar chickens on the 6th and 18th day of embryonic development using the MSP (methylation-specific PCR) method. Methylation was not detected in the promoter region of gene CDKN2B on the 6th and 18th day of embryonic development. As one of the five genes responsible for melanine activity in melanocytes and highly active, it can contribute to the production of this pigment. The present research broadens the current knowledge of the chicken epigenome and the mechanism of autosexing in birds.