2-Pyrrolidone synthesis from γ-aminobutyric acid produced by Lactobacillus brevis under solid-state fermentation utilizing toxic deoiled cottonseed cake
2-Pyrrolidone synthesis from γ-aminobutyric acid produced by Lactobacillus brevis under solid-state fermentation utilizing toxic deoiled cottonseed cake
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DOI：
10.1007/s00449-016-1683-9
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发表时间：
2017-01-01
影响因子：
3.8
通讯作者：
Khare, S. K.
中科院分区：
文献类型：
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作者：
Grewal, Jasneet;Khare, S. K.
γ-氨基丁酸（GABA）作为药物和食品添加剂以及生产2-吡咯烷酮（合成尼龙4的前体）的原料的需求不断增加。2-吡咯烷酮是一种石油化工和消耗储备，引起了对其生物基生产的关注。本文描述了以有毒的脱油棉籽饼（CSC）为底物，通过固态发酵（SSF）从短乳杆菌生产生物基经济的GABA。总的来说，由于存在有毒棉酚，棉籽饼的使用仍然受到限制。因此，在发酵过程中观察到的同时解毒也扩大了相对于生产其他增值化学品而言，将这种残留籽饼用于饲料用途的范围。优化SSF条件以获得最大GABA产量，即，19.7 mg/g，发酵第6天可获得GABA的CSC，同时棉酚降解率达70%。这种生物基GABA作为平台化学品的潜力在2-吡咯烷酮的合成中得到证实。因此，一个简单的和具有成本效益的战略，利用有毒的生物质已被开发作为替代化学合成路线。
There is an increasing demand of gamma-aminobutyric acid (GABA) as drug and food additive, as well as feedstock to produce 2-pyrrolidone, a precursor for the synthesis of nylon 4. 2-Pyrrolidone is a petrochemical and depleting reserve which raises concern for its bio-based production. The study herein describes bio-based economical GABA production from Lactobacillus brevis by solid-state fermentation (SSF) using toxic deoiled cottonseed cake (CSC) as substrate. In general, the use of cottonseed cake remains restricted due to the presence of toxic gossypols. Thus, simultaneous detoxification observed during fermentation also widens the scope of utilization of this residual seedcake for feed use vis-a-vis production of other value added chemicals. The SSF conditions were optimized for maximum GABA production, viz., 19.7 mg/g, CSC of GABA was obtained at 6th day of fermentation with 70 % degradation of gossypols simultaneously. The potential of this bio-based GABA as a platform chemical is demonstrated in the synthesis of 2-pyrrolidone. Thus, a simple and cost-effective strategy for utilizing toxic biomass has been developed as an alternate to chemical synthetic route.