Repeated exposure to low-level extremely low frequency-modulated microwaves affects cortex-hypothalamus interplay in freely moving rats: EEG study
Repeated exposure to low-level extremely low frequency-modulated microwaves affects cortex-hypothalamus interplay in freely moving rats: EEG study
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DOI：
10.3109/09553000903567938
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发表时间：
2010-04
影响因子：
2.6
通讯作者：
V. Vorobyov;B. Janać;V. Pešić;Z. Prolić
中科院分区：
文献类型：
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作者：
V. Vorobyov;B. Janać;V. Pešić;Z. Prolić
目的：目的比较极低频调制微波（ELF-MW）重复暴露对皮层和下丘脑脑电图（EEG）的影响。材料与方法：在10只自由活动的大鼠中，将碳电极植入皮层和背内侧下丘脑，在假暴露（5只大鼠）或混合假/MW暴露（5只大鼠）下连续5天研究EEG的平均频谱（0.5-30 Hz）。将大鼠暴露于ELF-MW（915 MHz，20 ms脉冲持续时间，<0.3 mW/cm 2，4 Hz），间歇性（1 min“开”，1 min“关”）10 min（比吸收率，SAR，平均<0.7 mW/g），每天数次，暴露前和暴露后各10 min。结果：在基线EEG中，皮层以3.2- 6.0Hz和17.8- 30.5Hz活动为主，下丘脑以6.0- 17.8Hz活动为主。这种皮质-下丘脑不平衡在假暴露时相对稳定，并且在除一个频率范围外的所有频率范围内对ELF-MW不敏感。ELF-MW增加β 2（17.8-30.5 Hz）水平在下丘脑比在皮层更大的程度上，导致显着减少的初始EEG之间的偏见。此外，在反复暴露于ELF-MW下的累积现象被揭示。结论：这些结果与反复低水平暴露于ELF-MW影响大脑功能的证据一致，并在分析潜在机制时提供了另一种方法。
Purpose: To compare the effects of repeated exposure to extremely low frequency-modulated microwaves (ELF-MW) on cortical and hypothalamic electroencephalograms (EEG). Materials and methods: In 10 freely moving rats with carbon electrodes implanted into the cortex and dorsomedial hypothalamus, averaged frequency spectra (0.5–30 Hz) of the EEG were studied for five consecutive days either under sham exposures (five rats) or under mixed sham/MW-exposures (five rats). The rats were exposed to ELF-MW (915 MHz, 20-ms pulse duration, ∼0.3 mW/cm2, 4 Hz) intermittently (1-min ‘On’, 1-min ‘Off’) for 10 min (specific absorption rate, SAR, ∼0.7 mW/g on average) several times per day, with 10-min pre- and post-exposure periods. Results: In baseline EEG, the activities of 3.2–6.0 Hz and 17.8–30.5 Hz dominated in the cortex and of 6.0–17.8 Hz in the hypothalamus. This cortical-hypothalamic imbalance was relatively stable at sham-exposures and insensitive to ELF-MW in all frequency ranges but one. ELF-MW increased the beta2 (17.8–30.5 Hz) level in the hypothalamus to a greater extent than in the cortex, causing significant diminishing of the initial EEG bias between them. Moreover, a cumulative phenomenon under repeated exposures to ELF-MW was revealed. Conclusions: These results are in line with evidence that repeated low-level exposure to ELF-MW affects brain functioning and provide an additional approach when analysing underlying mechanisms.