Effects of atrazine on metamorphosis, growth, and gonadal development in the green frog (Rana clamitans)
Effects of atrazine on metamorphosis, growth, and gonadal development in the green frog (Rana clamitans)
复制标题
DOI：
10.1080/15287390490443722
复制
发表时间：
2004-06-25
影响因子：
2.6
通讯作者：
Giesy, JP
中科院分区：
文献类型：
--
作者：
Coady, KK;Murphy, MB;Giesy, JP
从野外采集绿色蛙（Rana clamitans）的胚胎，并将其暴露于6种水性处理中的1种，持续273 d（2001年7月中旬至2002年4月中旬）。处理为0、10或25 μ g/L阿特拉津、0.005%乙醇（EtOH）或0.005%EtOH中携带的0.1 mg/L雌二醇或双氢睾酮。在静态更新系统中进行处理，大约每3天更换50%试验溶液。暴露期后，在淡水中饲养蝌蚪，直至变态或直至研究终止（第506天）。开始和完成变态的时间，特定阶段的死亡率，变态时的长度和重量，以及性腺的大体形态和组织学进行了检查。在环境相关浓度下，阿特拉津并不总是影响生长或变态。与对照组相比，幼虫期的长度是更大的蝌蚪暴露于10微克/L阿特拉津。然而，幼虫期的长度在对照组和25 μ g/L阿特拉津处理组的蝌蚪之间没有显着差异。无论是总性腺形态，也没有组织病理学的变性后青蛙的性腺显着改变阿特拉津暴露。本研究提供的证据表明，阿特拉津的环境相关浓度不会对生长或生殖发育的R。蛤蚌
Embryos of the green frog (Rana clamitans) were collected from the field and exposed to 1 of 6 water-borne treatments for 273 d (mid July 2001 to mid April 2002). The treatments were 0, 10, or 25 mug/L atrazine, 0.005% ethanol (EtOH), or 0.1 mg/L estradiol or dihydrotestosterone carried in 0.005% EtOH. Treatments were applied in a static renewal system with a 50% test solution replacement approximately every 3 d. Following the exposure period, tadpoles were reared in freshwater until metamorphosis or until study termination (at d 506). Time to initiate and complete metamorphosis, stage-specific mortality, length and weight at metamorphosis, and gross morphology and histology of the gonads were examined. At environmentally relevant concentrations, atrazine did not consistently affect growth or metamorphosis. Compared to controls, the length of the larval period was greater in tadpoles exposed to 10 mug/L atrazine. However, the length of the larval period was not markedly different between tadpoles in the control and 25 mug/L atrazine treatments. Neither gross gonadal morphology nor histopathology of the gonads in postmetamorphic frogs was significantly altered in response to atrazine exposure. This study provides evidence that environmentally relevant concentrations of atrazine do not adversely affect the growth or reproductive development of R. clamitans.