The structure of human skin collagen as studied with the electron microscope.
The structure of human skin collagen as studied with the electron microscope.
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DOI：
10.1084/jem.88.5.555
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发表时间：
1948-11
期刊：
影响因子：
--
通讯作者：
SCHMITT FO
中科院分区：
文献类型：
--
作者：
GROSS J;SCHMITT FO
1.本文用电镜观察了正常人真皮胶原纤维的结构。2.在观察条件下，原纤维的宽度范围为约700至1，400 A，其中1，000 A作为最频繁出现的值。它们几乎没有纵向磨损的倾向，这是肌腱原纤维的特征;当原纤维断裂时，通常发生在横向于轴线的平面上。3.在用磷钨酸染色或用铬或铂阴影的原纤维中观察到的轴向重复周期范围为约500至800埃，最大值出现在620至660埃之间。婴儿皮肤原纤维的平均周期与成人和老年皮肤无显著差异。4.根据不同的制备条件，intraperiod精细结构，在交叉带的形式，观察到不同的细节。通常观察到的最详细的图案包含六个特征密度和位置的带。5.干胶原纤维的阴影塑料复制品再现了阴影纤维中常见的大部分结构。湿原纤维的复制品比干原纤维显示出相当少的表面轮廓。玻璃上结缔组织碎片涂片的复制品显示了详细的结构，表明将这种技术应用于活检材料的可行性。6.婴儿皮肤与成人皮肤的不同之处在于具有相当大量的无定形材料，其中几乎没有与胶原原纤维强烈粘附。
1. The structure of the collagen fibrils of normal human skin corium has been investigated with the electron microscope. 2. Under the conditions of observation the fibrils ranged in width from about 700 to 1,400 A with 1,000 A as the value occurring most frequently. They showed little tendency to fray longitudinally as is characteristic of tendon fibrils; when fracture of fibrils occurred it was usually in planes transverse to the axis. 3. The axial repeating periods observed in fibrils stained with phosphotungstic acid or shadowed with chromium or platinum range from about 500 to 800 A, the maximum occurring between 620 and 660 A. The average period in fibrils from infant skin does not differ significantly from that of adult and aged skin. 4. Depending on conditions of preparation, intraperiod fine structure, in the form of cross-bands, was observed in varying detail. The most detailed pattern commonly observed contains six bands of characteristic density and position. 5. Shadowed plastic replicas of dried collagen fibrils reproduce much of the structure commonly seen in shadowed fibrils. Replicas of moist fibrils show considerably less surface contouring than do dried fibrils. Replicas from smears of connective tissue fragments on glass show detailed structure, indicating the feasibility of applying this technique to biopsy material. 6. Infant skin differs from adult skin in having considerably greater amounts of amorphous material, little of which is strongly adherent to the collagen fibrils.