Human muscle precursor cells give rise to functional satellite cells in vivo
Human muscle precursor cells give rise to functional satellite cells in vivo
复制标题
DOI：
10.1016/j.nmd.2007.04.009
复制
发表时间：
2007-08-01
影响因子：
2.8
通讯作者：
Morgan, Jennifer
中科院分区：
文献类型：
--
作者：
Ehrhardt, Janine;Brimah, Karima;Morgan, Jennifer
小鼠卫星细胞已被证明是功能性肌肉干细胞，因为它们能够再生骨骼肌并重建卫星细胞池。虽然人类肌肉前体细胞在移植到宿主小鼠肌肉后能够促进骨骼肌再生，但尚不确定它们是否也能产生功能性卫星细胞。在这里，我们将人胎儿肌肉前体细胞移植到C-5-/ γ -链-/ rag2宿主小鼠的冷冻损伤肌肉中。供体细胞产生的肌纤维在移植后可持续长达6个月。移植后4周，从肌肉中分离出携带卫星细胞的肌纤维。当置于培养中时，这些纤维中的一小部分产生了人类起源的肌肉前体细胞，这表明最初移植的细胞形成了卫星细胞以及再生的肌肉纤维。这些卫星细胞衍生的人类肌肉前体细胞在培养中扩增，并在移植到第二系列宿主小鼠后形成肌肉。这为人类和小鼠的肌肉前体细胞能够在体内形成功能性卫星细胞提供了证据。(C) 2007 Elsevier B.V.版权所有
Mouse satellite cells have been shown to be functional muscle stem cells, in that they are able to regenerate skeletal muscle and to reconstitute the satellite cell pool. Although human muscle precursor cells are able to contribute to skeletal muscle regeneration following transplantation into host mouse muscles, it is uncertain whether they also give rise to functional satellite cells.Here, we transplant human fetal muscle precursor cells into cryodamaged muscles in C-5-/gamma-chain-/Rag2-host mice. The donor cells gave rise to muscle fibres that persisted for up to 6 months after grafting. Isolated muscle fibres, bearing satellite cells, were prepared from muscles 4 weeks after grafting. When placed in culture, a small proportion of these fibres gave rise to muscle precursor cells of human origin, indicating that the originally grafted cells had formed satellite cells as well as regenerated muscle fibres. These satellite cell-derived human muscle precursor cells were expanded in culture and formed muscle following their transplantation into a second series of host mice. This provides evidence that human, as well as mouse, muscle precursor cells, are capable of forming functional satellite cells in vivo. (C) 2007 Elsevier B.V. All rights reserved.