Hypertrophic scar model in the rabbit ear: a reproducible model for studying scar tissue behavior with new observations on silicone gel sheeting for scar reduction
Hypertrophic scar model in the rabbit ear: a reproducible model for studying scar tissue behavior with new observations on silicone gel sheeting for scar reduction
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DOI：
10.1111/j.1524-475x.2007.00224.x
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发表时间：
2007-09-01
影响因子：
2.9
通讯作者：
Mustoe, Thomas A.
中科院分区：
文献类型：
--
作者：
Kloeters, Oliver;Tandara, Andrea;Mustoe, Thomas A.
增生性瘢痕是一个临床相关的问题，因为它可以美观毁容和功能削弱。缺乏动物模型阻碍了对发病机制的理解和新治疗策略的发展，因此在很大程度上是经验性的。我们团队在兔耳上开发了一种独特的增生性瘢痕(HS)模型。该模型在1个月的时间段内是可重现的、可量化的和可测量的。我们描述了这一模型的发展以及对不同治疗药物的可靠性和反应性，如转化生长因子-β阻断、硅胶封闭和胶原合成抑制剂的应用。此外，它还对硅胶薄膜闭塞治疗的作用机制进行了深入的研究，并最终表明表皮在HS的发展中起着关键作用。此外，我们将提供新的数据支持表皮的重要性，并进一步阐明硅胶薄膜的作用机制。当半封闭的聚氨酯薄膜放置一段时间后，疤痕的形成就会减少。与只有一层Tegaderm(R)的伤口相比，这种模型的HSS覆盖了硅胶片和五层Tega-derm(R)，显著减少了80%的疤痕。兔耳HS模型是研究瘢痕组织行为的一种高度可靠、灵敏、实用的模型。此外，我们的数据表明，闭塞的程度和持续时间对于减少瘢痕组织的形成是最重要的。
Hypertrophic scarring poses a clinically relevant problem as it can be cosmetically disfiguring and functionally debilitating. A lack of animal models has hindered an understanding of the pathogenesis and development of new treatment strategies therefore has largely been empiric. Our group has developed a unique hypertrophic scar (HS) model in the rabbit ear. The model has been reproducible, quantifiable, and measurable over a time period of I month. We describe the development as well as the reliability and responsiveness of this model to different therapeutic agents, such as TGF-beta blockade, silicone occlusion, and application of collagen-synthesis inhibitors. Moreover, it has given insights into the mechanism of action of silicone sheeting occlusive treatment and ultimately suggests that the epidermis plays a critical role in the development of HS. Additionally, we will present new data supporting the importance of the epidermis and further clarify the mechanism of action of silicone sheeting. When a semi-occlusive polyurethane film was left in place for an additional time period, scar formation was reduced. HSs of this model covered with silicone sheets and five layers of Tega-derm (R) showed a significant scar reduction by 80% compared with wounds with only one layer of Tegaderm (R). The HS model in the rabbit ear is a highly reliable, responsive, and practical model for studying scar tissue behavior. Furthermore, our data suggest that the degree and the duration of occlusion are most important for reducing scar tissue formation.