Modelling a risk classification of aneuploidy in human embryos using non-invasive morphokinetics
Modelling a risk classification of aneuploidy in human embryos using non-invasive morphokinetics
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DOI：
10.1016/j.rbmo.2013.02.006
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发表时间：
2013-05-01
影响因子：
4
通讯作者：
Hickman, Cristina Fontes Lindemann
中科院分区：
文献类型：
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作者：
Campbell, Alison;Fishel, Simon;Hickman, Cristina Fontes Lindemann
这项研究确定了非整倍体胚胎和整倍体胚胎之间的形态动力学变量是否不同，作为选择整倍体胚胎进行移植的潜在帮助。受精后，收集98枚胚泡的时间推移图像，并对其进行盲法倍性分析。将形态动力学变量与滋养外胚层活检后确定的倍性进行回顾性比较，并通过阵列比较基因组杂交或单核苷酸多态阵列进行分析。与整倍体胚胎(t(SC)的中位数79.7hPI，t(B)的中位数105.9 hPI相比，多个非整倍体胚胎在致密开始时延迟(t(SC)；受精后中位数85.1h；P=0.02)和达到完全囊胚期的时间(t(B)；中位数110.9 hpi，P=0.01)。与整倍体胚胎(中位数95.1HPI)相比，具有单个和多个非整倍体的胚胎(中位数分别为103.4 hpi和101.9 hpi，P=0.004和P=0.006)的胚泡发育延迟。整倍体和非整倍体胚胎在第一和第二细胞周期长度、第二和第三细胞周期的同步性、胚泡形成持续时间、2-细胞期多核和不规则分裂模式等方面没有显著差异。这种非侵入性倍性分类模型可用于避免选择非整倍体风险较高的胚胎，而选择风险较低的胚胎。(C)2013，爱思唯尔有限公司出版的《生殖健康有限公司》。版权所有。
This study determined whether morphokinetic variables between aneuploid and euploid embryos differ as a potential aid to select euploid embryos for transfer. Following insemination, EmbryoScope time-lapse images from 98 blastocysts were collected and analysed blinded to ploidy. The morphokinetic variables were retrospectively compared with ploidy, which was determined following trophectoderm biopsy and analysis by array comparative genomic hybridization or single-nucleotide polymorphic array. Multiple aneuploid embryos were delayed at the initiation of compaction (t(SC); median 85.1 hours post insemination (hpi); P = 0.02) and the time to reach full blastocyst stage (t(B); median 110.9 hpi, P = 0.01) compared with euploid embryos (t(SC) median 79.7 hpi, t(B) median 105.9 hpi). Embryos having single or multiple aneuploidy (median 103.4 hpi, P = 0.004 and 101.9 hpi, P = 0.006, respectively) had delayed initiation of blastulation compared with euploid embryos (median 95.1 hpi). No significant differences were observed in first or second cell-cycle length, synchrony of the second or third cell cycles, duration of blastulation, multinucleation at the 2-cell stage and irregular division patterns between euploid and aneuploid embryos. This non-invasive model for ploidy classification may be used to avoid selecting embryos with high risk of aneuploidy while selecting those with reduced risk. (C) 2013, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.