Microcystins (cyanobacteria hepatotoxins) bioaccumulation in fish and crustaceans from Sepetiba Bay (Brasil, RJ)
Microcystins (cyanobacteria hepatotoxins) bioaccumulation in fish and crustaceans from Sepetiba Bay (Brasil, RJ)
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DOI：
10.1016/s0041-0101(03)00144-2
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发表时间：
2003-09-01
期刊：
影响因子：
2.8
通讯作者：
Azevedo, SMFO
中科院分区：
文献类型：
--
作者：
Magalhaes, VF;Marinho, MM;Azevedo, SMFO
蓝藻水华的发生不仅造成严重的环境问题，而且其毒性菌株的产生也关系到人类的健康。水生动物可以生物积累微囊藻毒素（蓝细菌肝毒素），因此，除了水，摄入受污染的食物也会对人类健康造成风险。最近，WHO推荐了饮用水中微囊藻毒素（MCYSTs）的最大浓度，并确定了食用蓝藻产品的每日可耐受摄入量（TDI）为0.04 μ g（-1）kg（-1）天（-1）。Sepetiba湾位于里约热内卢的市辖区，Mangaratiba和Itaguai是渔业活动的重要场所。由于该地区的工业发展，该海湾受到不同的环境影响，增加了有机和工业污染。一株纳米浮游蓝藻集胞藻Synechocystis aquatilis f.产生MCYST的aquatilis已经被分离出来。在这项研究中，我们验证了MCYSTs存在于鱼类和甲壳类动物的肌肉组织中，这些动物在11个月期间每月在Sepetiba湾收获，以评估其摄入的潜在风险。使用ELISA微囊藻毒素板试剂盒（Microcystin Plate Kit）（RINROLOGIX INC（R））通过免疫测定技术分析MCYST，并且浓度表示为微囊藻毒素-LR当量。对悬浮物样品、水、肌肉组织的分析表明，所有样品中都存在这种氰毒素，并证实19%的动物样品高于世卫组织建议的人类食用限度。在螃蟹肌肉组织中发现的最大值为103.3 mug kg（-1）（TDI为0.52 mug kg（-1）天（-1）），最小值为0.25 mug kg（-1）（TDI为0.001 mug kg（-1）天（-1））。这些数据表明，尽管浓度较低，但Sepetiba湾的鱼类和甲壳类动物已经受到污染。我们强调，建议的限值是指健康成人。(C)2003年爱思唯尔有限公司保留所有战斗。
Blooms of cyanobacteria in water bodies cause serious environmental problems and the occurrence of toxic strains are also related with the human health. Aquatic animals could bioaccumulate microcystins (cyanobacteria hepatotoxins) and so, beyond water, the ingestion of contaminated food represents a human health risk. Recently, WHO recommended a maximum concentration of microcystins (MCYSTs) in drinking water and established the tolerable daily intake (TDI) for consumption of cyanobacteria products contends MCYSTs (0.04 mug(-1) kg(-1) day(-1)). Sepetiba Bay is located in the municipal districts of Rio de Janeiro, Mangaratiba and Itaguai being an important place of fishing activity. Due to the industrial development in the area, this bay is submitted to different environmental impacts, increasing the organic and industrial pollution. A strain of the nanoplanktonic cyanobacteria Synechocystis aquatilis f. aquatilis that produce MCYSTs was already isolated. In this study, we verified MCYSTs presence in muscle tissue of fish and crustaceans, which were harvested monthly in Sepetiba Bay during 11 months, in order to evaluate the potential risk of their ingestion. MCYSTs were analyzed by immunoassay techniques using the ELISA Microcystin Plate Kit (ENVIROLOGIX INC(R)) and the concentration were expressed as microcystin-LR equivalent. The analyses of seston samples, water, muscle tissues showed the presence of this cyanotoxin in all samples and it was verified that 19% of the animals' samples were above the limit recommended by WHO for human consumption. The maximum value found was of 103.3 mug kg(-1) (TDI 0.52 mug kg(-1) day(-1)) and the minimum, was 0.25 mug kg(-1) in crabs muscle tissue (TDI of 0.001 mug kg(-1) day(-1)). Such data demonstrate that, although in low concentrations, there is already a contamination of fish and crustaceans from Sepetiba Bay. We highlight that the recommended limit refers to healthy adult. (C) 2003 Elsevier Ltd. All fights reserved.