THICKNESS OF ADHERENT MUCUS GEL ON COLONIC MUCOSA IN HUMANS AND ITS RELEVANCE TO COLITIS
THICKNESS OF ADHERENT MUCUS GEL ON COLONIC MUCOSA IN HUMANS AND ITS RELEVANCE TO COLITIS
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DOI：
10.1136/gut.35.3.353
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发表时间：
1994-03-01
期刊：
影响因子：
24.5
通讯作者：
RHODES, J
中科院分区：
文献类型：
--
作者：
PULLAN, RD;THOMAS, GAO;RHODES, J
我们测量了46例结肠癌患者手术切除标本结肠粘膜表面黏液凝胶的厚度；右结肠12例，左结肠17例，直肠21例。此外，还检查了17例溃疡性结肠炎患者和15例克罗恩病患者的标本。在对照组中，通过相衬照明在特殊制备的切片上很容易看到连续的粘液层。右、左结肠和直肠的平均值分别为107（48）、134（68）和155(54)，右结肠和直肠的平均值差异有统计学意义（p=0.015）。溃疡性结肠炎的数值变化较大，急性炎症区粘膜黏液层脱落。相比之下，克罗恩病的厚度值正常或大于正常-右结肠190（83）与107 (48),p=0.0093。一系列的验证实验描述了用于测量粘液厚度的方法。讨论了黏液在炎症性肠病发病机制中的可能作用。
The thickness of adherent mucus gel on the surface of colonic mucosa was measured in surgically resected specimens from 46 'control' patients most of whom had carcinoma of the colon; 12 were from right colon, 17 left colon, and 21 from rectum. In addition specimens were examined from 17 patients with ulcerative colitis and 15 patients with Crohn's disease. In controls a continuous layer of mucus was readily seen on specially prepared sections viewed by phase contrast illumination. Mean values for right and left colon and rectum were 107 (48), 134 (68), and 155 (54) mum respectively with a significant difference between right colon and rectum (p=0.015). Values in ulcerative colitis showed greater variation and in those areas with acute inflammation mucosa was denuded of the mucus layer. In contrast, values for Crohn's disease were normal or greater than normal in thickness - right colon 190 (83) mum compared with 107 48 mum, p=0.0093. A series of validation experiments are described for the method used to measure mucus thickness. The possible role of mucus in the pathogenesis of inflammatory bowel disease is discussed.