Quantitative measurement of optical parameters of cell lines 5-8F and 6-10B using polarization sensitive optical coherence tomography.
Quantitative measurement of optical parameters of cell lines 5-8F and 6-10B using polarization sensitive optical coherence tomography.
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DOI：
10.1016/j.abb.2012.04.009
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发表时间：
2012-06
影响因子：
3.9
通讯作者：
Jianghua Li;Bingling Chen;Yong Du;Lifeng Yang;Yun-Fei Xia;Yonghong He;Song-hao Liu;Changshui Chen
中科院分区：
文献类型：
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作者：
Jianghua Li;Bingling Chen;Yong Du;Lifeng Yang;Yun-Fei Xia;Yonghong He;Song-hao Liu;Changshui Chen
目的是测试偏振敏感光学相干断层扫描(PS-OCT)能否区分典型的鼻咽癌细胞系5-8F(高致瘤性和转移性)和6-10B(低致瘤性和低转移性)。我们用PS-OCT对两种低细胞分化的鼻咽癌细胞系5-8F和6-10B颗粒进行成像，然后基于多重散射模型从A扫描线中提取衰减系数和各向异性的光学参数，并比较它们的相位延迟。5-8F和6-10B微丸的散射系数分别为μS=10.91±0.45和μS=11.33±0.27 cm−1，各向异性因子分别为g=0.900±0.013和g=0.885±0.008。而6-10B的位相延迟比5-8F略快。这些结果表明，PS-OCT可以区分这两种细胞系，并具有潜在的鼻咽癌组织分型能力。
The aim was to test whether the typical NPC cell lines of 5-8F (high tumorigenesis and metastasis) and 6-10B (low tumorigenesis and metastasis) could be differentiated by polarization sensitive optical coherence tomography (PS-OCT). We imaged the two types of low cellular differentiated NPC cell lines 5-8F and 6-10B pellets using PS-OCT; then extracted the optical parameters of attenuate coefficient and anisotropy from the A-scan lines based on the multiple scattering model; and compared their phase retardation. The fitting scattering coefficients were μs=10.91±0.45 and μs=11.33±0.27cm−1for 5-8F and 6-10B pellets (p<0.05), respectively; and the anisotropy factors were g=0.900±0.013 and g=0.885±0.008 for 5-8F and 6-10B pellets (p<0.01), respectively. While the phase retardation of 6-10B was a little faster than 5-8F. These results indicated that PS-OCT could differentiate the two cell lines, and had the potential ability for typing the tissue of NPC.