IDENTIFICATION OF THE HEPATOCYTE GROWTH-FACTOR RECEPTOR AS THE C-MET PROTOONCOGENE PRODUCT
IDENTIFICATION OF THE HEPATOCYTE GROWTH-FACTOR RECEPTOR AS THE C-MET PROTOONCOGENE PRODUCT
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DOI：
10.1126/science.1846706
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发表时间：
1991-02-15
期刊：
影响因子：
56.9
通讯作者：
AARONSON, SA
中科院分区：
文献类型：
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作者：
BOTTARO, DP;RUBIN, JS;AARONSON, SA
肝细胞生长因子(HGF)是一种纤溶酶原样蛋白，被认为是肝脏再生的体液介质。通过免疫印迹分析，观察到一个145kodalton的酪氨酸磷酸酶对HGF处理完整的靶细胞的快速反应，它是原癌基因产物c-met的β亚基，是一种跨越膜的酪氨酸激酶。I-125标记的配体与c-met抗体识别的适当大小的细胞蛋白的共价交联直接确立了c-met产物为HGF的细胞表面受体。
Hepatocyte growth factor (HGF) is a plasminogen-like protein thought to be a humoral mediator of liver regeneration. A 145-kilodalton tyrosyl phosphoprotein observed in rapid response to HGF treatment of intact target cells was identified by immunoblot analysis as the beta-subunit of the c-met proto-oncogene product, a membrane-spanning tyrosine kinase. Covalent cross-linking of I-125-labeled ligand to cellular proteins of appropriate size that were recognized by antibodies to c-met directly established the c-met product as the cell-surface receptor for HGF.