Inactivation of the flagellin gene of Salmonella enterica serotype enteritidis strongly reduces invasion into differentiated Caco-2 cells.
Inactivation of the flagellin gene of Salmonella enterica serotype enteritidis strongly reduces invasion into differentiated Caco-2 cells.
复制标题
肠炎沙门氏菌血清型肠炎的鞭毛蛋白基因失活可强烈减少对分化 Caco-2 细胞的侵袭。
DOI：
10.1111/j.1574-6968.2000.tb09058.x
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发表时间：
2000
影响因子：
2.1
通讯作者：
Wim Gaastra
中科院分区：
文献类型：
--
作者：
Fons J.A.M. Asten;H. J. Hendriks;Jos F.J.G. Koninkx;B. A. Zeijst;Wim Gaastra
通过破坏鞭毛蛋白基因（fliC）构建了肠道沙门氏菌血清型肠道沙门氏菌的无鞭毛突变体。北方印迹分析表明该突变不影响下游fliU基因的表达。用分化的Caco-2细胞进行的感染实验显示，突变体的侵袭性比野生型菌株低约50倍，而细菌粘附不受影响。具有完整fliC拷贝的突变体的互补恢复了鞭毛形成和有效的细菌入侵。我们的数据表明，S.肠球菌血清型肠球菌对于Caco-2细胞的侵袭是必需的。
A nonflagellated mutant of Salmonella enterica serotype Enteritidis was constructed by disrupting the flagellin gene (fliC). Northern blot analysis indicated that the mutation did not affect expression of the downstream fliU gene. Infection experiments with differentiated Caco-2 cells revealed that the mutant was about 50-fold less invasive than the wild-type strain, while bacterial adherence was unaffected. Complementation of the mutant with an intact fliC copy restored flagella formation and efficient bacterial invasion. Our data demonstrate that the fliC gene of S. enterica serotype Enteritidis is essential for the invasion of Caco-2 cells.