CTFFIND4: Fast and accurate defocus estimation from electron micrographs
CTFFIND4: Fast and accurate defocus estimation from electron micrographs
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DOI：
10.1016/j.jsb.2015.08.008
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发表时间：
2015-11-01
影响因子：
3
通讯作者：
Grigorieff, Nikolaus
中科院分区：
文献类型：
--
作者：
Rohou, Alexis;Grigorieff, Nikolaus
CTFFIND是一个广泛使用的程序估计物镜透镜离焦参数从透射电子显微镜。散焦参数估计拟合的显微镜的对比度传递函数（CTF）的图像的振幅谱的模型。在这里，我们描述的算法，使其显着更快，更适合使用现代技术，如剂量分割和相位板收集的图像的修改。我们表明，这个新版本保留了原算法的准确性，同时允许更高的吞吐量。我们还描述了作为空间频率的函数的拟合的质量的测量，并建议这可以用于定义CTF振荡成功建模的最高分辨率。(C)2015 Elsevier Inc. All rights reserved.
CTFFIND is a widely-used program for the estimation of objective lens defocus parameters from transmission electron micrographs. Defocus parameters are estimated by fitting a model of the microscope's contrast transfer function (CTF) to an image's amplitude spectrum. Here we describe modifications to the algorithm which make it significantly faster and more suitable for use with images collected using modern technologies such as dose fractionation and phase plates. We show that this new version preserves the accuracy of the original algorithm while allowing for higher throughput. We also describe a measure of the quality of the fit as a function of spatial frequency and suggest this can be used to define the highest resolution at which CTF oscillations were successfully modeled. (C) 2015 Elsevier Inc. All rights reserved.