APPLICATION OF 3-(2-FUROYL)QUINOLINE-2-CARBALDEHYDE AS A FLUOROGENIC REAGENT FOR THE ANALYSIS OF PRIMARY AMINES BY LIQUID-CHROMATOGRAPHY WITH LASER-INDUCED FLUORESCENCE DETECTION
APPLICATION OF 3-(2-FUROYL)QUINOLINE-2-CARBALDEHYDE AS A FLUOROGENIC REAGENT FOR THE ANALYSIS OF PRIMARY AMINES BY LIQUID-CHROMATOGRAPHY WITH LASER-INDUCED FLUORESCENCE DETECTION
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DOI：
10.1016/s0021-9673(00)97002-x
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发表时间：
1990-01-19
期刊：
影响因子：
--
通讯作者：
NOVOTNY, M
中科院分区：
文献类型：
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作者：
BEALE, SC;HSIEH, YZ;NOVOTNY, M
合成了3-（2-呋喃甲酰基）喹啉-2-甲醛（C3 HCA），并对其进行了表征。该试剂在与伯胺反应时形成高荧光异吲哚。研究了异吲哚的光谱性质和最佳反应条件。的实用程序的高灵敏度分析的氨基酸的激光诱导荧光与氩离子激光器被证明。
3-(2-Furoyl)quinoline-2-carbaldehyde (FQCA) has been synthesized and characterized as a fluorogenic derivatizing reagent for liquid chromatography. The reagent forms highly fluorescent isoindoles upon reaction with primary amines. The spectral properties and optimum reaction conditions for the formation of isoindoles have been investigated. The utility of FQCA for the high-sensitivity analysis of amino acids by laser-induced fluorescence with an argon-ion laser is demonstrated.