Angiotensin II AT-1A receptor immunolabeling in rat medial nucleus tractus solitarius neurons: Subcellular targeting and relationships with catecholamines
Angiotensin II AT-1A receptor immunolabeling in rat medial nucleus tractus solitarius neurons: Subcellular targeting and relationships with catecholamines
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DOI：
10.1016/j.neuroscience.2004.08.057
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发表时间：
2005-01-01
期刊：
影响因子：
3.3
通讯作者：
Pickel, VM
中科院分区：
文献类型：
--
作者：
Glass, MJ;Huang, J;Pickel, VM
血管紧张素Ⅱ AT-1A受体（AT-1A）是孤束内侧核（mNTS）中血管紧张素Ⅱ（ANG Ⅱ）降压作用的主要介质。AT-1A受体在表面或细胞内位点的定位是其信号传导特性的重要决定因素，包括细胞间或分泌内通讯。然而，这种蛋白质的空间定位，特别是在小的远端或中间大小的树突的mNTS神经元，是未知的。在mNTS内，ANG II和儿茶酚胺在自主神经功能的调节中相互作用;然而，尚不清楚AT-1A受体是否存在于含有儿茶酚胺的树突中的功能位点，或者是否与含有儿茶酚胺的轴突末端接触。我们比较了表面和胞内分布的AT-1A受体在树突状过程中的mNTS使用免疫金电子显微镜结合免疫过氧化物酶标记酪氨酸羟化酶（TH）和形态计量学分析。在所有AT-1A标记的树突状细胞的配置文件中崩溃，免疫金标记是更频繁的细胞内的网站相比，质膜。小（
The angiotensin II AT-1A receptor (AT-1A) is the major mediator of the hypertensive actions of angiotensin II (ANG II) in the medial nucleus of the solitary tract (mNTS). The localization of the AT-1A receptor at surface or intracellular sites is an important determinant of its signaling properties, including intercellular or intracrine communication. However, the spatial localization of this protein, particularly within small distal or intermediate size dendrites of mNTS neurons, is unknown. Within the mNTS, ANG II and catecholamines interact in the regulation of autonomic function; however, it is unknown if AT-1A receptors are present at functional sites in catecholamine containing dendrites, or are contacted by catecholamine containing axon terminals. We compared surface and intracellular distributions of the AT-1A receptor in dendritic processes from the mNTS using immunogold electron microscopy in conjunction with immunoperoxidase labeling for tyrosine hydroxylase (TH) and morphometric analysis. Collapsed across all AT-1A-labeled dendritic profiles, immunogold labeling was more frequent in intracellular sites as compared with the plasma membrane. Small (