Microsatellite resources for Passeridae species: a predicted microsatellite map of the house sparrow Passer domesticus
Microsatellite resources for Passeridae species: a predicted microsatellite map of the house sparrow Passer domesticus
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DOI：
10.1111/j.1755-0998.2012.03115.x
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发表时间：
2012-05-01
影响因子：
7.7
通讯作者：
Burke, Terry
中科院分区：
文献类型：
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作者：
Dawson, Deborah A.;Horsburgh, Gavin J.;Burke, Terry
我们确定了对家雀（Passer domesticus）具有潜在用途的微卫星序列，并分配了它们的预测基因组位置。这些序列包括新分离的家雀位点，我们充分的特点。许多新分离的位点在雀科的其他两个物种中表现出多态性：Berthelots pitAnthus berthelotii和斑胸草雀Taeniopygia guttata。总之，我们确定了179个微卫星标记，这些标记要么直接从麻雀中分离出来，要么在麻雀中具有已知的实用性。其中67个标记是从我们从家雀基因组文库中分离的独特序列中设计的。这些新的标记结合了36个家雀标记分离的其他研究和76个标记分离的其他雀形目物种，但已知是多态性的家雀。我们利用序列同源性来分配这些位点在组装斑胸草雀基因组中的染色体位置。134个位点被分配到25个不同的常染色体和8个位点的Z染色体。对已知性别家雀的37个新位点进行基因型检测，发现一个W连锁位点和一个额外的Z连锁位点。基因座Pdo mu 2，以前报道为常染色体，被发现是Z连锁。这些位点使创建强大的和具有成本效益的家雀多重引物组的人口和亲子关系的研究。它们可用于建立家雀连锁图谱，并将有助于雀形目物种数量性状基因座的鉴定。
We identified microsatellite sequences of potential utility in the house sparrow (Passer domesticus) and assigned their predicted genome locations. These sequences included newly isolated house sparrow loci, which we fully characterized. Many of the newly isolated loci were polymorphic in two other species of Passeridae: Berthelots pipit Anthus berthelotii and zebra finch Taeniopygia guttata. In total, we identified 179 microsatellite markers that were either isolated directly from, or are of known utility in, the house sparrow. Sixty-seven of these markers were designed from unique sequences that we isolated from a house sparrow genomic library. These new markers were combined with 36 house sparrow markers isolated by other studies and 76 markers isolated from other passerine species but known to be polymorphic in the house sparrow. We utilized sequence homology to assign chromosomal locations for these loci in the assembled zebra finch genome. One hundred and thirty-four loci were assigned to 25 different autosomes and eight loci to the Z chromosome. Examination of the genotypes of known-sex house sparrows for 37 of the new loci revealed a W-linked locus and an additional Z-linked locus. Locus Pdo mu 2, previously reported as autosomal, was found to be Z-linked. These loci enable the creation of powerful and cost-effective house sparrow multiplex primer sets for population and parentage studies. They can be used to create a house sparrow linkage map and will aid the identification of quantitative trait loci in passerine species.