Ca2(+)-dependent amylase secretion from pancreatic acinar cells occurs without activation of phospholipase C linked G-proteins.
Ca2(+)-dependent amylase secretion from pancreatic acinar cells occurs without activation of phospholipase C linked G-proteins.
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DOI：
10.1016/0006-291x(91)91450-q
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发表时间：
1991-01
影响因子：
3.1
通讯作者：
P. Padfield;T. Ding;J. Jamieson
中科院分区：
文献类型：
--
作者：
P. Padfield;T. Ding;J. Jamieson
我们观察了鸟嘌呤核苷酸对SLO通透性大鼠胰腺腺泡细胞分泌钙依赖的淀粉酶的影响。GTPγS(100μM)刺激依赖钙的淀粉酶的释放，使其对钙的EC50从1.4μM降至0.8μM，而国内生产总值(1 MM)和dGDP(1 MM)则抑制最大的钙依赖分泌反应。对IP_3释放的测定表明，Ca~(2+)刺激不会增加磷脂酶C(PLC)的活性，因此GDP和dGDP必须通过一种不同于PLC连接的GP的GTP结合蛋白来发挥抑制作用。
We have examined the influence of guanine nucleotides on Ca2+-dependent amylase secretion from SLO permeabilized rat pancreatic acini. GTPγS (100μM) stimulated Ca2+-dependent amylase release, decreasing the EC50for Ca2+from 1.4 to 0.8μM. By contrast, GDP (1mM) and dGDP (1mM) inhibited the maximal Ca2+-dependent secretory response. Measurement of IP3liberation showed that Ca2+stimulation did not increase the activity of phospholipase C (PLC) postulated to be linked to a G-protein termed Gp; GDP and dGDP must therefore be exerting their inhibitory action via a GTP-binding protein distinct from the PLC-linked Gp.