Expression patterns of the RGS9-1 anchoring protein R9AP in the chicken and mouse suggest multiple roles in the nervous system
Expression patterns of the RGS9-1 anchoring protein R9AP in the chicken and mouse suggest multiple roles in the nervous system
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DOI：
10.1016/s1044-7431(03)00231-8
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发表时间：
2003-11-01
影响因子：
3.5
通讯作者：
Heller, S
中科院分区：
文献类型：
--
作者：
Keresztes, G;Mutai, H;Heller, S
在视网膜感光器中，G 蛋白信号传导的持续时间受到 GTP 酶激活蛋白 RGS9-1 的严格调节。 RGS9-1 通过与跨膜蛋白 R9AP 结合而锚定在光感受器外节的盘膜上。在这里，我们报道了从内耳 cDNA 文库克隆鸡 R9AP 以及从视网膜文库分离鼠 R9AP cDNA。在鸡中，R9AP 似乎在多种神经元组织中表达，特别是在内耳毛细胞、光感受器和背根神经节神经元等感觉细胞中。在小鼠中，R9AP 主要在光感受器中检测到，但在中枢神经系统的其他区域也有微弱表达。 R9AP 在光感受器之外的表达使我们研究了 R9AP 除了锚定 RGS9-1 之外的潜在替代作用，我们发现该蛋白与 SNARE 蛋白家族成员的序列同源性和结构相似性。在体外测定中，鸡和小鼠 R9AP 的表达干扰指示蛋白的细胞内运输，表明该蛋白可能在靶向中发挥更积极的作用。 GTP 酶激活蛋白作用于特定的膜区室。 (C) 2003 Elsevier Inc. 保留所有权利。
In retinal photoreceptors, the duration of G protein signalling is tightly regulated by the GTPase-activating protein RGS9-1. RGS9-1 is anchored to the disk membranes of photoreceptor outer segments by association with the membrane-spanning protein R9AP. Here we report the cloning of chicken R9AP from an inner ear cDNA library and the isolation of a murine R9AP cDNA from a retinal library. In the chicken, R9AP appears to be expressed in a variety of neuronal tissues, particularly in sensory cells including inner ear hair cells, photoreceptors, and dorsal root ganglion neurons. In the mouse, R9AP is detectable predominantly in photoreceptors, but it is also weakly expressed in other areas of the central nervous system. The expression of R9AP beyond photoreceptors led us to examine potential alternative roles for R9AP besides anchoring RGS9-1 and we found sequence homology and structural similarity of the protein with members of the SNARE protein family. Expression of chicken and mouse R9AP interfered with intracellular trafficking of an indicator protein in an in vitro assay, suggesting a more active role of the protein, possibly in targeting. GTPase-activating proteins to specific membranous compartments. (C) 2003 Elsevier Inc. All rights reserved.