Mucin Genes Expression in the Intestine of Crohn's Disease Patients: a Systematic Review and Meta-analysis.
Mucin Genes Expression in the Intestine of Crohn's Disease Patients: a Systematic Review and Meta-analysis.
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DOI：
10.15403/jgld.2014.1121.253.niv
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发表时间：
2016-09
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影响因子：
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通讯作者：
Y. Niv
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文献类型：
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作者：
Y. Niv
背景与目的肠道粘液层是肠道微生物与粘膜之间的主要屏障，在机体防御机制中起着重要作用。先前的研究不能建立克罗恩病（CD）患者粘蛋白基因表达的一致结果。在这项荟萃分析中，我们观察了CD患者的粘蛋白表达，并将其与健康对照进行了比较。方法检索英文医学文献，分析CD患者回肠和结肠粘膜中粘蛋白的表达，并与正常粘膜进行比较。纳入病例对照研究。使用Comprehensive荟萃分析软件进行荟萃分析。计算合并优势比和95%置信区间。结果我们找到了160个合格的研究。20项研究因在动物中进行或没有全文而被拒绝，134项研究因语言、社论、综述文章或重复而被排除。截至2015年12月31日，我们还剩下来自4个国家的6项病例对照研究，这些研究符合入选标准。未显示显著异质性：Q = 149.256，df（Q）= 40.00，I2= 73.2%（小于75%）。我们发现CD患者的总粘蛋白表达降低了34%（比值比0.660，95% CI 0.486-0.897，P = 0.008）。我们还发现CD患者中MUC 5AC、MUC 5 B和MUC 7的表达显著降低。结论CD患者的粘蛋白表达较健康对照组明显降低。
BACKGROUND AND AIMS The mucus layer of the intestinal tract is the main barrier between luminal microbes and the mucosa, and has an essential role in the body defense mechanisms. Previous research could not establish consistent results for mucin genes expression in Crohn's disease (CD) patients. In this meta-analysis we looked at the mucin expression in CD patients and compared it with healthy controls. METHOD English medical literature searches were conducted for mucin expression in the mucosa of the ileum and colon of CD patients and compared it with normal mucosa. Case-control studies were included. Meta-analysis was performed by using Comprehensive meta-anaslysis software. Pooled odds ratios and 95% confidence intervals were calculated. RESULTS We found 160 eligible studies. Twenty studies were rejected because they have been performed in animals or did not have full text, and 134 studies were excluded because of language, being editorials, review articles, or because of duplications. We were left with 6 case-control studies from 4 countries that fulfilled the inclusion criteria, published till 31.12.2015. No significant heterogeneity was demonstrated: Q = 149.256, df (Q) = 40.00, I2= 73.2% (less than 75%). We found a decrease of 34% in the total mucin expression in CD patients (Odds Ratio 0.660, 95% CI 0.486-0.897, P = 0.008). We also found a significantly decreased expression in CD patients for MUC5AC, MUC5B and MUC7. CONCLUSION We demonstrated a global decrease in mucin expression in CD patients when compared with healthy controls.