miR-30a suppresses osteosarcoma proliferation and metastasis by downregulating MEF2D expression.
miR-30a suppresses osteosarcoma proliferation and metastasis by downregulating MEF2D expression.
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miR-30a通过下调MEF2D表达抑制骨肉瘤增殖和转移
DOI：
10.2147/ott.s102430
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发表时间：
2018
影响因子：
4
通讯作者：
Yang D
中科院分区：
文献类型：
--
作者：
Du L;Chen T;Zhao K;Yang D
许多研究表明microRNAs（miRNAs）在癌症的发生和发展中起着至关重要的作用。miRNA-30a（miR-30a）是miR-30家族的成员之一，与多种癌症有关。然而，miR-30a在骨肉瘤中的作用仍不清楚。在本研究中，我们发现miR-30 a在骨肉瘤组织和细胞系中显著下调，通过使用定量实时聚合酶链反应（qRT-PCR）。此外，miR-30 a在体外可抑制癌细胞的生长、迁移和侵袭。此外，生物信息学的miRNA靶点预测和荧光素酶报告基因分析表明，MEF2D是miR-30a的直接靶点。通过RT-PCR和Western blotting检测，miR-30 a能够降低MEF2D的mRNA和蛋白表达。有趣的是，MEF2D过表达部分逆转了miR-30 a降低的骨肉瘤细胞增殖、迁移和侵袭，表明miR-30 a抑制骨肉瘤细胞增殖和转移部分由MEF2D抑制介导。总之，我们的研究表明，miR-30a通过靶向MEF2D在骨肉瘤中发挥肿瘤抑制剂的作用，为骨肉瘤提供了一种有前途的预后生物标志物和治疗策略。
Many studies have revealed that microRNAs (miRNAs) play crucial roles in cancer development and progression. miRNA-30a (miR-30a), as a member of the miR-30 family, has been implicated in various cancers. However, the role of miR-30a in osteosarcoma remains unclear. In the current study, we found that miR-30a was significantly downregulated in osteosarcoma tissues and cell lines by using quantitative real-time polymerase chain reaction (qRT-PCR). In addition, miR-30a could inhibit cancer cell growth, migration, and invasion in vitro. Furthermore, bioinformatics of miRNA target prediction and luciferase reporter assay indicated that MEF2D is a direct target of miR-30a. miR-30a was able to reduce the mRNA and protein expression of MEF2D as assessed using RT-PCR and Western blotting assay. Interestingly, overexpression of MEF2D partially reversed the miR-30a-reduced cell proliferation, migration, and invasion of osteosarcoma cell, indicating that miR-30a suppresses osteosarcoma cell proliferation and metastasis partially mediated by inhibition of MEF2D. Overall, our study demonstrated that miR-30a functions as a tumor suppressor by targeting MEF2D in osteosarcoma, providing a promising prognostic biomarker and a therapeutic strategy for osteosarcoma.