Interaction of fibronectin (FN) cell binding fragments and interleukin-8 (IL-8) in regulating neutrophil chemotaxis.
Interaction of fibronectin (FN) cell binding fragments and interleukin-8 (IL-8) in regulating neutrophil chemotaxis.
复制标题
纤连蛋白 (FN) 细胞结合片段和白细胞介素 8 (IL-8) 在调节中性粒细胞趋化性中的相互作用。
DOI：
10.1006/bbrc.1994.2723
复制
发表时间：
1994
影响因子：
3.1
通讯作者：
Frewin,MB
中科院分区：
文献类型：
--
作者：
Gudewicz,PW;Heinel,LA;Stanton,K;Singleton,D;Frewin,MB
本研究使用 Neuroprobe 微室研究了 FN 片段在体外调节 IL-8 介导的中性粒细胞趋化性中可能的相互作用。将人中性粒细胞悬浮液与纯化的 FN 片段或含有 RGD 的肽一起孵育，并允许其响应趋化活性浓度的人重组 IL-8 而迁移。含有 RGD 序列或 RGD 肽 (GRGDSP) 的 120 kD FN 片段抑制 IL-8 介导的中性粒细胞趋化性；然而，这些 RGD 肽不会抑制中性粒细胞对其他趋化剂的趋化反应。此外，不包含RGD序列的FN片段对IL-8介导的趋化性没有影响。这些数据表明，在存在 FN 细胞结合片段的情况下，中性粒细胞响应 IL-8 的定向迁移受到抑制，并且可能代表了终止组织损伤区域中性粒细胞迁移的局部机制。
This study investigated the possible interaction of FN fragments in regulating IL-8-mediated neutrophil chemotaxis in vitro using Neuroprobe microchambers. Human neutrophil suspensions were incubated with purified FN fragments or an RGD-containing peptide and allowed to migrate in response to chemotactically active concentrations of human recombinant IL-8. The 120-kD fragment of FN containing the RGD sequence or an RGD peptide (GRGDSP) inhibited IL-8-mediated neutrophil chemotaxis; however, these RGD peptides did not inhibit neutrophil chemotaxis in response to other chemotactic agents. Furthermore, FN fragments not containing the RGD sequence had no effect on IL-8-mediated chemotaxis. These data suggest that directed migration of neutrophils in response to IL-8 is inhibited in the presence of cell-binding fragments of FN and may represent a local mechanism for terminating neutrophil migration at areas of tissue injury.