Direct patterning of mammalian cells onto porous tissue engineering substrates using agarose stamps
Direct patterning of mammalian cells onto porous tissue engineering substrates using agarose stamps
复制标题
DOI：
10.1016/j.biomaterials.2005.05.001
复制
发表时间：
2005-12-01
期刊：
影响因子：
14
通讯作者：
Langer, R
中科院分区：
文献类型：
--
作者：
Stevens, MM;Mayer, M;Langer, R
本文介绍了简单，廉价，和潜在的通用方法，用于产生模式的哺乳动物细胞的多孔支架组织工程使用复制打印。圆形图案（直径：200、700和1000 μ m）的人成骨细胞直接从地形图案化的琼脂糖印模转移到多孔羟基磷灰石支架上或纤连蛋白包被的载玻片上。水凝胶印模的使用提供了“湿的”生物相容性表面，并在图案化过程中保持了吸附在印模上的细胞的活力。用细胞悬浮液上墨一次的印模允许基底的重复图案化。直接冲压人成骨细胞（以及潜在的其他哺乳动物细胞）可用于控制打印在多孔组织工程基底上的细胞图案的大小、间距和几何形状。这种方法可用于控制支架的空间侵入，促进细胞的分层组织，以及控制细胞-细胞相互作用作为细胞表型保存的一个步骤。(c)2005爱思唯尔有限公司保留所有权利。
This paper describes simple, inexpensive, and potentially generic methodology for generating patterns of mammalian cells on porous scaffolds for tissue engineering using replica printing. Circular patterns (diameter: 200, 700, and 1000 mu m) of human osteoblasts were transferred directly from topographically patterned agarose stamps onto porous hydroxyapatite scaffolds or onto fibronectin-coated glass slides. The use of hydrogel stamps provided a "wet", biocompatible surface and maintained the viability of cells adsorbed on stamps during the patterning process. stamps inked once with suspensions of cells allowed the repeated patterning of substrates. Direct stamping of human osteoblasts (and, potentially other mammalian cells) can be used to control the size, spacing, and geometry of patterns of cells printed on porous tissue engineering substrates. This approach may find use in controlling the spatial invasion of scaffolds, promoting the hierarchical organization of cells, and in controlling cell-cell interactions as a step in preservation of phenotypes of cells. (c) 2005 Elsevier Ltd. All rights reserved.