NADPH oxidase subunit, gp91phox homologue, preferentially expressed in human colon epithelial cells
NADPH oxidase subunit, gp91phox homologue, preferentially expressed in human colon epithelial cells
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DOI：
10.1016/s0378-1119(00)00258-4
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发表时间：
2000-08-22
期刊：
影响因子：
3.5
通讯作者：
Fukumoto, M
中科院分区：
文献类型：
--
作者：
Kikuchi, H;Hikage, M;Fukumoto, M
NADPH氧化酶是在多种细胞中发现的一组质膜相关酶。它们以NADPH为电子供体，通过氧的单电子还原催化超氧化物（O-2（-））的产生。为了表征该酶的表达，使用来自DNA数据库的与表达序列标签（EST）相关的信息，从人结肠癌细胞系Caco 2和人胎肾的cDNA克隆了NADPH氧化酶催化亚基的两个同源物gp 91（phox），氨基酸同一性分别为58%（gp 91 -2）和56%（gp 91 -3），针对外周血白细胞中发现的NADPH氧化酶的催化亚基（gp 91 -1/gp 91（phox））。用逆转录-聚合酶链反应（RT-PCR）方法检测到gp 91 -2的信使RNA主要存在于人成年组织中的结肠（以及肾脏和前列腺）、人胎儿组织中的胸腺以及癌细胞系（HepG 2和Caco 2）中。gp 91 -3在胎儿肾和癌细胞系（HepG 2）中检测到表达，但在成人组织中完全没有（通过RT-PCR方法）。原位杂交显示，gp 91 -2定位于成人结肠吸收上皮细胞。gp 91 -2和gp 91 -3在外周血白细胞中均不表达。(C)2000 Elsevier Science B. V.保留所有权利。
The NADPH oxidases are a group of plasma membrane-associated enzymes found in a variety of cells. They catalyze the production of superoxide (O-2(-)) by a one-electron reduction of oxygen, using NADPH as the electron donor. To characterize the expression of this enzyme, two homologues of the NADPH oxidase catalytic subunit, gp91(phox), were cloned from the cDNAs of a human colon cancer cell line, Caco2, and human fetal kidney, using information relating to an expressed sequence tag (EST) from a DNA database, Amino acid identity was 58% (gp91-2) and 56% (gp91-3), respectively, against the catalytic subunit (gp91-1/gp91(phox)) of the NADPH oxidase found in peripheral blood leukocytes. Using the reverse transcription-polymerase chain reaction (RT-PCR) method, the messenger RNA of gp91-2 was detected mainly in the colon (and also in kidney and prostate) among human adult tissues, in the thymus among human fetal tissues, and in the cancer cell lines (HepG2 and Caco2). An expression of gp91-3 was detected in the fetal kidney, and in the cancer cell line (HepG2), but not at all in adult tissues (by the RT-PCR method). In situ hybridization revealed that gp91-2 is located in the absorptive epithelial cells of the adult colon. Neither gp91-2 nor gp91-3 was expressed in peripheral blood leukocytes. (C) 2000 Elsevier Science B.V. All rights reserved.