Evaluation of renal oxygen saturation using photoacoustic imaging for the early prediction of chronic renal function in a model of ischemia-induced acute kidney injury
Evaluation of renal oxygen saturation using photoacoustic imaging for the early prediction of chronic renal function in a model of ischemia-induced acute kidney injury
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DOI：
10.1371/journal.pone.0206461
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发表时间：
2018-12-17
期刊：
影响因子：
3.7
通讯作者：
Gabata, Toshifumi
中科院分区：
文献类型：
--
作者：
Okumura, Kenichiro;Matsumoto, Junichi;Gabata, Toshifumi
目的评价光声成像技术在缺血性急性肾损伤后肾血氧饱和度变化中的应用，并将其与组织学和血清肾功能水平进行比较。材料与方法夹持左肾蒂诱导C57BI/6小鼠急性肾损伤，轻度肾损伤35 min（14只），重度肾损伤50 min（13只）。在诱导前和诱导后48 h内的5个时间点测量血氧饱和度，从诱导后4 h开始。第28天测定血氧饱和度、组织学评分、肾体积、24 h肌酐清除率和血清尿素氮。使用Mann-Whitney u检验和Dunn多重比较评估组间差异。血氧饱和度与测量变量之间的关系采用Spearman相关法进行评估。根据半肾切除术后24 h的血氧饱和度值构建接受者操作者特征曲线，预测慢性肾功能。结果轻度肾损伤组诱导后24 h血氧饱和度高于重度肾损伤组（分别为73.7%和66.9%）
PurposeTo evaluate the utility of photoacoustic imaging in measuring changes in renal oxygen saturation after ischemia-induced acute kidney injury, and to compare these measurements with histological findings and serum levels of kidney function.Material and MethodsAcute kidney injury was induced by clamping the left renal pedicle in C57BI/6 mice, with a 35-min ischemic period used to induce mild renal injury (14 mice) and a 50-min period for severe injury (13 mice). The oxygen saturation was measured before induction, and at 5 time-points over the first 48 h after induction, starting at 4 h after induction. Oxygen saturation, histological score, kidney volume, and the 24 h creatinine clearance rate and serum blood urea nitrogen were also measured on day 28. Between-group differences were evaluated using a Mann-Whitney U-test and Dunn's multiple comparisons. The association between oxygen saturation and measured variables was evaluated using Spearman's correlation. A receiver operator characteristic curve was constructed from oxygen saturation values at 24 h after heminephrectomy to predict chronic renal function.ResultsThe oxygen saturation was higher in the mild than severe renal injury group at 24 h after induction (73.7% and 66.9%, respectively, P