Biological and prognostic value of ETV5 in high-grade serous ovarian cancer.
Biological and prognostic value of ETV5 in high-grade serous ovarian cancer.
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DOI：
10.1186/s13048-021-00899-6
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发表时间：
2021-11-04
影响因子：
4
通讯作者：
Tao L
中科院分区：
文献类型：
--
作者：
Zhang L;Fu R;Liu P;Wang L;Liang W;Zou H;Jia W;Tao L
已知ETS转录因子作为参与各种生物过程的基因表达的正或负调节剂。ETS变异转录因子5（ETV 5）是ETS家族的重要成员，主要作为潜在的癌基因在多种恶性肿瘤中发挥作用。然而，ETV 5在高级别浆液性卵巢癌（HGSOC）中的作用和机制尚未阐明。采用实时定量聚合酶链反应（qRT-PCR）检测87例HGSOC组织和35例正常输卵管组织中ETV 5 mRNA的表达。采用Western blotting和qRT-PCR方法检测ETV 5在6种卵巢癌细胞株和人胚胎细胞株中的蛋白和mRNA表达。ETV 5在HGSOC细胞系中的敲低或过表达、细胞计数试剂盒-8、集落形成和transwell测定用于检测HGSOC细胞增殖、侵袭和迁移能力。采用卡方检验分析HGSOC患者的临床病理特征。采用Kaplan-Meier法进行生存分析，并采用log-rank检验分析ETV 5表达与HGSOC患者预后的相关性。使用考克斯回归模型进行单变量和多变量分析，以确定相关临床协变量的独立显著性。生物信息学分析表明ETV 5在OC中的表达显著上调（p < 0.05）。qRT-PCR结果显示ETV 5在HGSOC组织中的表达显著高于输卵管组织（p < 0.05）。qRT-PCR和western blotting检测表明ETV 5在OC细胞系中相对高表达。ETV 5过表达与HGSOC患者的不良生存率呈正相关，因此使其成为HGSOC进展的高危因素。此外，ETV 5促进HGSOC细胞的增殖、迁移和侵袭能力。ETV 5在HGSOC中具有致癌作用，可以作为临床上有效的生物标志物来确定HGSOC患者的预后。在线版本包含补充材料，可通过10.1186/s13048-021-00899-6获得。
ETS transcription factors are known to act as either positive or negative regulators of the expression of genes involved in various biological processes. It was reported that ETS variant transcription factor 5 (ETV5), a key member of the ETS family, mainly plays a role as an potential oncogene in various malignant tumors. However, the role and mechanism of ETV5 in high-grade serous ovarian cancer (HGSOC) have not been elucidated. Quantitative real-time polymerase chain reaction (qRT-PCR) assay was used to detect ETV5 messenger ribonucleic acid (mRNA) expression in 87 HGSOC tissues and 35 normal fallopian tube tissues. Western blotting and qRT-PCR were used to detect the protein and mRNA expression of ETV5 in six ovarian cancer (OC) and human embryonic cell lines. Knockdown or overexpression of ETV5 in HGSOC cell lines, Cell Counting Kit-8, colony formation, and transwell assays were used to detect HGSOC cell proliferation, invasion, and migration capabilities. The chi-square test was used to analyze the clinicopathological characteristics of HGSOC patients. Survival analysis was performed using the Kaplan-Meier method, and the log-rank test was used to analyze the correlation between ETV5 expression and HGSOC patient prognosis. Univariate and multivariate analyses using the Cox regression model were conducted to determine the independent significance of relevant clinical covariates. Bioinformatic analysis demonstrated that ETV5 expression was significantly upregulated in OC (p < 0.05). qRT-PCR showed that ETV5 was significantly overexpressed in HGSOC tissues than in fallopian tube tissues (p < 0.05). qRT-PCR and western blotting assays demonstrated that ETV5 was relatively highly expressed in OC cell lines. ETV5 overexpression was positively associated with poor survival in HGSOC patients, therefore making it a high-risk factor for HGSOC progression. Furthermore, ETV5 promoted the proliferation, migration, and invasion capabilities of HGSOC cells. ETV5 has a carcinogenic effect in HGSOC and can be used as a clinically effective biomarker to determine the prognosis of HGSOC patients. The online version contains supplementary material available at 10.1186/s13048-021-00899-6.
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