Hepatoprotective Effects of Selenium-Enriched Probiotics Supplementation on Heat-Stressed Wistar Rat Through Anti-Inflammatory and Antioxidant Effects
Hepatoprotective Effects of Selenium-Enriched Probiotics Supplementation on Heat-Stressed Wistar Rat Through Anti-Inflammatory and Antioxidant Effects
复制标题
富硒益生菌对热应激大鼠肝脏的抗炎和抗氧化保护作用
DOI：
10.1007/s12011-020-02475-3
复制
发表时间：
2021-09-01
影响因子：
3.9
通讯作者：
Chen, Xingxiang
中科院分区：
文献类型：
--
作者：
Malyar, Rahmani Mohammad;Naseri, Emal;Chen, Xingxiang
The purpose of this study was to elucidate the effects of selenium-enriched probiotics on the liver of heat-stressed Wistar rats. Ten-week-old male rats were assigned to four groups: control (Con); high temperature (HT); high temperature plus probiotics (HT+P: 10(11) CFU/mL Lactobacillus acidophilus and 10(9) CFU/mL Saccharomyces cerevisiae); or high temperature plus selenium-enriched probiotics (HT+SeP: 0.3 mg/kg Se, 10(11) CFU/mL L. acidophilus and 10(9) CFU/mL S. cerevisiae). The HT, HT+P, and HT+SeP groups were maintained at higher ambient temperature (40-42 degrees C), while the control group was kept at room temperature (25 degrees C). After 42 days of thermal exposure, blood and liver tissues were collected and analyzed for morphological and molecular markers of liver physiology. The body weight of rats in the HT group decreased but liver weight and live index were increased. Histological examination showed dilation of liver sinusoids and congestion of interstitial veins in HT group. Moreover, the histomorphology of the liver in HT+P and HT+SeP groups was restored, and the serum AST, ALT, ALP, LDH, and hepatic MDA level decreased significantly, but the serum total protein level and the liver SOD, T-AOC, and GSH-PX activities were increased significantly relative to the HT group. In addition, the mRNA level of Gpx1, SOD1, Nrf2, and Bcl-2 was significantly increased, while the expression level of Bax, IL-6, TNF-alpha, COX-2, NF-kappa B, alpha -SMA, TGF beta 1, Collagen I, HSP70, and HSP90 was significantly decreased in liver tissues after SeP supplementation. We concluded that SeP can protect Wistar rats from oxidative stress, inflammation, apoptosis, and liver fibrosis induced by heat stress.
