Expressing Status and Correlation of ARID1A and Histone H2B on Breast Cancer.
Expressing Status and Correlation of ARID1A and Histone H2B on Breast Cancer.
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ARID1A 和组蛋白 H2B 在乳腺癌中的表达状况和相关性。
DOI：
10.1155/2016/7593787
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发表时间：
2016
影响因子：
--
通讯作者：
Zhang W
中科院分区：
文献类型：
--
作者：
Wu Y;Gu Y;Guo S;Dai Q;Zhang W
ARID1A是重要的癌症相关基因之一，通过改变染色质结构调控某些基因的转录。ARID1A基因失活突变和表达降低已在几种癌症中报道。组蛋白H2B是染色质的主要组成部分，由HIST1H2BE编码。本研究的目的是评估ARID1A和H2B的表达状况及其与乳腺癌的相关性。分析了ARID1A和H2B基因在Oncomine数据库中的表达谱。采用乳腺癌组织芯片免疫组化检测ARID1A和H2B的表达。结果发现ARID1A表达不一致，而在Oncomine数据库的5个数据集中，有4个数据集的HIST1H2BE表达升高。有15例（20%）乳腺癌患者ARID1A阳性。75例乳腺癌中H2B蛋白高表达58例（77.3%），低表达17例（22.7%）。所有ARID1A表达病例均与H2B高表达重叠。在15例ARID1A与H2B共表达的病例中，浸润性导管癌13例，粘液癌2例。我们的研究结果提示ARID1A基因可能参与某些亚型乳腺癌的癌变过程。
ARID1A is one of the important cancer-related genes and regulates transcription of certain genes by altering chromatin structure. Inactivated mutations and decreased expression of ARID1A gene have been reported in several kinds of cancer. Histone H2B is a major component of chromatin and encoded by HIST1H2BE. The goal of the study is to evaluate expressing status of ARID1A and H2B as well as their correlation on breast cancer. Gene expression profiles of ARID1A and H2B on Oncomine database are analyzed. Tissue microarray of breast cancer was used for examination of ARID1A and H2B expression by immunohistochemistry. As a result, the disagreement of ARID1A expression was found, while HIST1H2BE expression is elevated in 4 out of 5 datasets on Oncomine database. There were 15 cases (20%) of breast cancers that were positive for ARID1A. Fifty-eight out of 75 cases of breast cancer (77.3%) were highly expressed for H2B protein and 17 cases (22.7%) were low expressed for H2B protein. All cases with ARID1A expression are overlapped with H2B high expression. Among 15 cases with ARID1A and H2B coexpression, 13 are invasive ductal carcinoma and 2 are mucinous carcinoma. Our results indicate that ARID1A gene may be involved in carcinogenesis of some subtypes of breast cancer.