Method for continuous measurements of renal sympathetic nerve activity and cardiovascular function during exercise in rats
Method for continuous measurements of renal sympathetic nerve activity and cardiovascular function during exercise in rats
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DOI：
10.1113/eph8702281
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发表时间：
2002-01-01
影响因子：
2.7
通讯作者：
Hayashida, Y
中科院分区：
文献类型：
--
作者：
Miki, K;Kosho, A;Hayashida, Y
在运动过程中，交感神经系统被认为在调节心血管功能方面起着重要作用。然而，只有少数的直接测量交感神经活动在整个身体的动态运动已经尝试。在本研究中，我们建立了一种方法，可以常规测量大鼠在跑步机时肾交感神经活动（RSNA）和心血管功能。我们在手术前训练Wistar大鼠在跑步机上跑步一周。至少在实验前2天，在无菌条件下植入记录RSNA、心电图、肌电图的电极，以及测量全身动脉和中心静脉压的导管。术后1-3天、4-7天和8-10天，80%、60%和40%的患者信噪比满意。在跑步机运动过程中，成功地记录了RSNA，没有受到外界噪音的污染。跑步机运动导致RSNA在0.5 min时突然增加82%，然后在跑步机运动开始后的5-30 min期间达到接近40%的稳定水平。该实验模型为研究大鼠动态运动过程中心血管功能调控的神经机制提供了基础。
The sympathetic nervous system is believed to play a major role in regulating cardiovascular function during exercise. However, only a few direct measurements of sympathetic nervous activity during whole body dynamic exercise have been attempted. In the present study, we have established a method to allow routine measurement of renal sympathetic nerve activity (RSNA) and cardiovascular function during treadmill exercise in rats. We trained Wistar rats to run on the treadmill for a week before the surgery. At least 2 days before the experiment, electrodes for recording RSNA, electrocardiogram and electromyogram, and catheters for the measurements of systemic arterial and central venous pressures were implanted under aseptic conditions. Satisfactory signal to noise ratios were obtained in 80%, 60% and 40% of the group at 1-3 days, 4-7 days and 8-10 days after the surgery, respectively. RSNA was successfully recorded without contamination by external noise during treadmill exercise. Treadmill exercise resulted in an abrupt increase in RSNA, by 82% at 0.5 min, and then reached a stable level of similar to40% during the period of 5-30 min after the onset of treadmill exercise. This experimental model allows us to study the neural mechanisms involved in the regulation of cardiovascular function during dynamic exercise in rats.