Gene expression profiling of mid to late secretory phase endometrial biopsies from women with menstrual complaint
Gene expression profiling of mid to late secretory phase endometrial biopsies from women with menstrual complaint
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DOI：
10.1016/j.ajog.2006.05.002
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发表时间：
2006-08-01
影响因子：
9.8
通讯作者：
Ghazal, Peter
中科院分区：
文献类型：
--
作者：
Critchley, Hilary O. D.;Robertson, Kevin A.;Ghazal, Peter
目的：本研究的目的是测试定量高通量分子筛选是否可用于探测人类子宫内膜，并启动分子诊断工具的开发，该工具有可能用于确定月经投诉女性的治疗靶点。收集10例大出血患者的子宫内膜，通过组织学测定和血清孕酮浓度将其分为月经周期的中期或晚期分泌期。提取总RNA，使用高密度寡核苷酸阵列评估基因活性。结果：统计学检验鉴定出83个“签名”基因，其表达水平区分月经周期的中期和晚期分泌阶段。结果表明，子宫内膜是一种复杂的异质组织，可用于高通量分子分析，这项工作为将来将分子谱应用于临床诊断提供了进一步的支持。(c)2006 Mosby，Inc. All rights reserved.
Objective: The purpose of this study was to test whether a quantitative high-throughput molecular screen can be used to probe human endometrium and initiate the development of molecular diagnostic tools with potential for identification of therapeutic targets in women with menstrual complaints.Study design: Endometrium was collected from 10 patients with complaint of heavy bleeding, classified into mid or late secretory phase of the menstrual cycle by histologic dating and serum progesterone concentration. Total RNA was extracted and gene activity assessed using high-density oligonucleotide arrays.Results: Statistical testing identified 83 'signature' genes whose expression levels differentiated the mid and late secretory phases of the menstrual cycle.Conclusion: The results show that the endometrium, a complex heterogeneous tissue, is amenable to high-throughput molecular analyses and this work provides further support for the future application of molecular profiling to clinical diagnosis. (c) 2006 Mosby, Inc. All rights reserved.