Miniature spectrometers for biochemical analysis

Miniature spectrometers for biochemical analysis
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DOI:
10.1117/12.269957
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发表时间:
1997-03
期刊:
--
影响因子:
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通讯作者:
G. Yee;N. Maluf;P. Hing;M. Albin;G. Kovacs
G. Yee;N. Maluf;P. Hing;M. Albin;G. Kovacs
中科院分区:
其他
文献类型:
--
作者:
G. Yee;N. Maluf;P. Hing;M. Albin;G. Kovacs

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微型光谱仪的演示,通过安装在CCD成像器件的微加工衍射光栅。测试了两种实现方式:一种用于高色散和高灵敏度应用,另一种用于低成本消费者应用。第一个系统显示了1.7 nm/像素的色散和74.4的分辨率,用于感兴趣的带宽。这些设备的自由光谱范围被设计为在可见光范围内,用于该特定应用。系统的衍射效率为63%。第二,低成本的系统表现出的色散和分辨率分别为2.55纳米/像素和69.8。这些规格与传统的低端商用光谱仪相当。结果显示其在生化分析中的应用。通过添加微加工透镜和创建专门的计算机生成的光栅来压缩和整形光谱信号来寻求优化。
Miniature spectrometers were demonstrated by mounting micromachined diffraction gratings onto CCD imaging devices. Two implementations were tested: one for high dispersion and high sensitivity applications, and the other for low-cost consumer applications. The first system showed a dispersion of 1.7 nm/pixel and a resolution of 74.4 for the bandwidth of interest. The free spectral range of these devices was designed to be in the visible range for this particular application. The diffraction efficiency of the system was 63%. The second, low-cost system demonstrated a dispersion and resolution of 2.55 nm/pixel and 69.8 respectively. These specifications are comparable to that of a conventional, low-end commercial spectrometer. Results are shown for their applications in biochemical analysis. Optimization was sought by adding micromachined lenses and creating specialized, computer generated gratings to compress and shape the spectral signal.