Determination of 3-chloro-L-tyrosine as a novel indicator of chlorine poisoning utilizing gas chromatography-mass spectrometric analysis
Determination of 3-chloro-L-tyrosine as a novel indicator of chlorine poisoning utilizing gas chromatography-mass spectrometric analysis
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DOI：
10.1016/j.legalmed.2020.101782
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发表时间：
2020-11-01
期刊：
影响因子：
1.5
通讯作者：
Nogami, Makoto
中科院分区：
文献类型：
--
作者：
Nishio, Tadashi;Toukairin, Yoko;Nogami, Makoto
化学战、工业和家庭事故中都会发生氯气暴露。在法医学中，次氯酸钠洗涤剂与强酸洗涤剂混合产生的氯气不可忽视，因为存在自杀法（NaC1O + 2HCl -> NaCl + H2O + Cl-2）的可能性。尽管典型的尸检结果是在氯暴露中获得的，例如肺水肿，但不存在有用的生物标志物。在本研究中，我们开发了一种利用气相色谱-质谱 (GC-MS) 分析血液中 3-氯-L-酪氨酸 (Cl-Tyr) 的方法，作为氯中毒的新型标志物。 Cl-Tyr 使用蛋白质沉淀和阳离子交换固相萃取进行纯化，通过硅烷化试剂进行衍生化并进行 GC-MS 分析。定量范围为10-200 ng/mL，重现性良好。我们应用开发的方法对疑似氯中毒的尸检样本中的 Cl-Tyr 进行分析，在左心血中检测到 59.7 ng/mL Cl-Tyr。据我们所知，这是第一份测定氯中毒人体尸检样本中氯化生物分子的报告。
Chlorine gas exposure occurs in chemical warfare, industrial and household accidents. In forensic science, the generation of chlorine gas by mixing sodium hypochlorite detergent and strong acid detergent cannot be overlooked because of the possibility of suicide method (NaC1O + 2HCl -> NaCl + H2O + Cl-2). Though typical autopsy findings are obtained in chlorine exposure, such as pulmonary edema, useful biomarkers don't exist. In this research, we developed an analytical method of 3-chloro-L-tyrosine (Cl-Tyr) in blood as a novel marker of chlorine poisoning utilizing gas chromatography-mass spectrometry (GC-MS). Cl-Tyr was purified using protein precipitation and cation-exchange solid phase extraction, derivatized by the silylation agent and subjected to GC-MS. The quantification range was 10-200 ng/mL and good reproducibility was obtained. We applied the developed method to analyze Cl-Tyr in autopsy sample, which is suspected of chlorine poisoning, and detected 59.7 ng/mL Cl-Tyr in left heart blood. To our knowledge, this is the first report of determination of the chlorinated biomolecule in the human autopsy sample from chlorine poisoning.