Comprehensive Analysis of Therapy-Related Messenger RNAs and Long Noncoding RNAs as Novel Biomarkers for Advanced Colorectal Cancer
Comprehensive Analysis of Therapy-Related Messenger RNAs and Long Noncoding RNAs as Novel Biomarkers for Advanced Colorectal Cancer
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DOI：
10.3389/fgene.2019.00803
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发表时间：
2019-11-20
影响因子：
3.7
通讯作者：
Wang, Yongpeng
中科院分区：
文献类型：
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作者：
Li, Jibin;Ma, Siping;Wang, Yongpeng
结直肠癌（CRC）是最常见的人类癌症之一。然而，CRC进展的机制仍然难以捉摸。本研究通过分析公共数据集 GSE94104，确定了局部晚期 CRC 的治疗前活检和治疗后切除之间不同表达的信使 RNA (mRNA)、长非编码 RNA (lncRNA) 和小核仁 RNA (snoRNA)。我们在治疗前和治疗后的局部晚期 CRC 样本中发现了 427 个失调的 mRNA、4 个失调的 lncRNA 和 19 个失调的 snoRNA。通过构建蛋白质-蛋白质相互作用网络和共表达网络，我们鉴定了10个关键mRNA、4个关键lncRNA和7个关键snoRNA。生物信息学分析显示，治疗相关的 mRNA 与核小体组装、重组 DNA 染色质沉默、基因表达负调控和 DNA 复制相关。治疗相关的lncRNA与细胞粘附、细胞外基质组织、血管生成和姐妹染色单体凝聚力相关。此外，与治疗相关的 snoRNA 与 DNA 复制、核小体组装和端粒组织相关。我们认为这项研究为识别 CRC 的新型生物标志物提供了有用的信息。
Colorectal cancer (CRC) is one of the most common types of human cancers. However, the mechanisms underlying CRC progression remained elusive. This study identified differently expressed messenger RNAs (mRNAs), long noncoding RNAs (lncRNAs), and small nucleolar RNAs (snoRNAs) between pre-therapeutic biopsies and post-therapeutic resections of locally advanced CRC by analyzing a public dataset, GSE94104. We identified 427 dysregulated mRNAs, 4 dysregulated lncRNAs, and 19 dysregulated snoRNAs between pre- and post-therapeutic locally advanced CRC samples. By constructing a protein-protein interaction network and co-expressing networks, we identified 10 key mRNAs, 4 key lncRNAs, and 7 key snoRNAs. Bioinformatics analysis showed therapy-related mRNAs were associated with nucleosome assembly, chromatin silencing at recombinant DNA, negative regulation of gene expression, and DNA replication. Therapy-related lncRNAs were associated with cell adhesion, extracellular matrix organization, angiogenesis, and sister chromatid cohesion. In addition, therapy-related snoRNAs were associated with DNA replication, nucleosome assembly, and telomere organization. We thought this study provided useful information for identifying novel biomarkers for CRC.