The mitosis-to-interphase transition is coordinated by cross talk between the SIN and MOR pathways in Schizosaccharomyces pombe.
The mitosis-to-interphase transition is coordinated by cross talk between the SIN and MOR pathways in Schizosaccharomyces pombe.
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DOI：
10.1083/jcb.201002055
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发表时间：
2010-09-06
期刊：
影响因子：
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通讯作者：
McCollum D
中科院分区：
文献类型：
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作者：
Ray S;Kume K;Gupta S;Ge W;Balasubramanian M;Hirata D;McCollum D
SIN通路通过阻止莫尔通路组分Orb6的激活来阻断胞质分裂期间不适当的肌动蛋白重排。在有丝分裂和间期之间的过渡过程中调节细胞骨架重塑的机制知之甚少。在分裂酵母中，莫尔通路促进肌动蛋白在间期向细胞尖端极化，而SIN信号通路驱动肌动球蛋白环组装和胞质分裂。我们发现SIN通过干扰Nak1激酶介导的最下游莫尔组分NDR家族激酶Orb6的激活来抑制有丝分裂中的莫尔信号传导。莫尔的失活可能是SIN的关键功能，因为莫尔信号的减弱挽救了SIN突变体的细胞动力学缺陷，并允许弱SIN信号触发异位胞质分裂。此外，当细胞分裂装置受损时，不能抑制莫尔是有毒的。总之，我们的研究结果揭示了SIN和莫尔通路之间的相互拮抗关系，这对于完成胞质分裂和协调有丝分裂到间期过渡的细胞骨架重塑非常重要。
The SIN pathway blocks inappropriate actin rearrangements during cytokinesis by preventing activation of the MOR pathway component Orb6. The mechanisms that regulate cytoskeletal remodeling during the transition between mitosis and interphase are poorly understood. In fission yeast the MOR pathway promotes actin polarization to cell tips in interphase, whereas the SIN signaling pathway drives actomyosin ring assembly and cytokinesis. We show that the SIN inhibits MOR signaling in mitosis by interfering with Nak1 kinase-mediated activation of the most downstream MOR component, the NDR family kinase Orb6. Inactivation of the MOR may be a key function of the SIN because attenuation of MOR signaling rescued the cytokinetic defects of SIN mutants and allowed weak SIN signaling to trigger ectopic cytokinesis. Furthermore, failure to inhibit the MOR is toxic when the cell division apparatus is compromised. Together, our results reveal a mutually antagonistic relationship between the SIN and MOR pathways, which is important for completion of cytokinesis and coordination of cytoskeletal remodeling at the mitosis-to-interphase transition.