Tissue Engineering for Regeneration of the Tracheal Epithelium
Tissue Engineering for Regeneration of the Tracheal Epithelium
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DOI：
10.1177/000348940611500704
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发表时间：
2006-07
期刊：
影响因子：
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通讯作者：
Yukio Nomoto;Teruhisa Suzuki;Y. Tada;Ken Kobayashi;M. Miyake;A. Hazama;I. Wada;S. Kanemaru;Tatsuo Nakamura;K. Omori
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文献类型：
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作者：
Yukio Nomoto;Teruhisa Suzuki;Y. Tada;Ken Kobayashi;M. Miyake;A. Hazama;I. Wada;S. Kanemaru;Tatsuo Nakamura;K. Omori
目的：目前已成功应用于人体的人工气管上皮化缓慢是一个问题。本研究的目的是开发一种方法来再生上皮的表面上的这种人工气管。方法：在体外研究中，将分离的大鼠气管上皮细胞接种在胶原海绵上分层的胶原凝胶上。进行组织学和免疫组化检查。在体内研究中，我们将绿色荧光蛋白阳性气管上皮细胞移植到正常大鼠气管缺损处。术后3、7、14、30天分别行组织学和免疫组化检查。结果：在体外研究中，可以观察到3层-上皮、凝胶和海绵。上皮细胞表达细胞角蛋白14、细胞角蛋白18和闭合蛋白。在体内实验中，术后3d人工气管被上皮覆盖，上皮由单层或双层鳞状上皮分化为柱状纤毛上皮。术后第3天出现绿色荧光蛋白阳性细胞。结论：我们认为本实验所用的方法是一种有效的人工气管表面上皮再生的方法。通过进一步的实验，该方法应适合于临床应用。
Objectives: The slowness of epithelialization on the artificial trachea that has been successfully used in humans is a problem. The purpose of this study was to develop a way to regenerate the epithelium on the surface of this artificial trachea. Methods: In an in vitro study, isolated rat tracheal epithelial cells were seeded on a collagenous gel that was stratified on a collagenous sponge. Histologic and immunohistochemical examinations were made. In an in vivo study, we transplanted grafts with green fluorescent protein–positive tracheal epithelial cells onto the tracheal defects of normal rats. At 3, 7, 14, and 30 days after the operation, histologic and immunohistochemical examinations were made. Results: In the in vitro study, the 3 layers — the epithelium, gel, and sponge — could be observed. The epithelium expressed cytokeratin 14, cytokeratin 18, and occludin. In the in vivo study, the artificial trachea was covered with epithelium at 3 days after operation, and then the epithelium differentiated from single- or double-stratified squamous epithelium into columnar ciliated epithelium. Green fluorescent protein–positive cells were found 3 days after operation. Conclusions: We believe that the method used in our experiment is an effective way to regenerate the epithelium on the surface of an artificial trachea. With further experimentation, this method should be suitable for clinical application.