Pseudomonas aeruginosa clearance in mice: comparison of tissue, strain, and corticosteroid effects.
Pseudomonas aeruginosa clearance in mice: comparison of tissue, strain, and corticosteroid effects.
复制标题
小鼠铜绿假单胞菌清除率：组织、菌株和皮质类固醇效应的比较。
DOI：
10.1128/iai.43.3.901-905.1984
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发表时间：
1984
影响因子：
3.1
通讯作者：
Pesanti,EL
中科院分区：
文献类型：
--
作者：
Nugent,KM;Cox,CD;Pesanti,EL
我们使用小鼠模型来确定慢性皮质类固醇治疗是否对不同组织中的细菌清除过程具有一致的影响。在口服强的松龙2周后，用铜绿假单胞菌PAO1菌株或PAO1毒力衍生物(IP-8和IT-10)攻击瑞士Webster小鼠。慢性糖皮质激素治疗延迟了PAO1菌株从腹膜腔的清除。然而，类固醇治疗并没有减少中性粒细胞的流入，没有降低体内中性粒细胞的吞噬能力，也没有改变腹膜渗出细胞的体外杀菌活性。尽管中性粒细胞流入与对照小鼠相似，但在接受类固醇治疗的小鼠中，毒力分离株(IP-8和IT-10)在腹膜腔内复制。与异常的腹膜清除相反，在气雾剂攻击后，所有菌株都从对照组和类固醇治疗组的小鼠肺中迅速清除。类固醇治疗组小鼠的中性粒细胞流入支气管肺泡腔的量比对照组大。所有的小鼠(对照组和类固醇治疗组)都在这些挑战中存活下来，除了一些类固醇治疗的小鼠被IP-8感染。这些结果表明，与下呼吸道相比，慢性类固醇治疗对腹膜表面细菌清除过程的影响更大。腹膜清除改变的原因尚不清楚，但不能用中性粒细胞迁移到炎性刺激的减少来解释。因此，与慢性皮质类固醇治疗相关的感染风险似乎既取决于组织，也取决于细菌菌株的毒力，可能反映了中性粒细胞迁移以外的清除过程的变化。
We used a murine model to determine whether chronic corticosteroid therapy has uniform effects on bacterial clearance processes in different tissues. After a 2-week regimen of oral prednisolone, Swiss Webster mice were challenged with Pseudomonas aeruginosa strain PAO1 or virulent derivatives of PAO1 (IP-8 and IT-10). Chronic corticosteroid therapy delayed the clearance of strain PAO1 from the peritoneal cavity. However, steroid treatment did not reduce the neutrophil influx, did not reduce the in vivo phagocytic capacity of neutrophils, and did not alter bactericidal activity of peritoneal exudate cells in vitro. Virulent isolates (IP-8 and IT-10) replicated in the peritoneal cavity in steroid-treated mice even though the neutrophil influx was similar to control mice. In contrast to the abnormal peritoneal clearance, all strains were rapidly cleared from the lungs of control and steroid-treated mice after aerosol challenge. Neutrophil influx into bronchoalveolar spaces was greater in steroid-treated mice than in control mice. All mice (control and steroid treated) survived these challenges, except for some steroid-treated mice infected intraperitoneally with IP-8. These results demonstrate that chronic steroid therapy alters bacterial clearance processes on the peritoneal surfaces to a greater extent than in the lower respiratory tract. The explanation for this altered clearance in the peritoneum is unclear, but cannot be explained by reductions in neutrophil migration to inflammatory stimuli. Therefore, the infectious risk associated with chronic corticosteroid therapy appears to depend on both the tissue and the virulence of the bacterial strain and may reflect alterations in clearance processes other than neutrophil migration.