Epstein-Barr Virus Type 2 Infects T Cells in Healthy Kenyan Children
Epstein-Barr Virus Type 2 Infects T Cells in Healthy Kenyan Children
复制标题
DOI：
10.1093/infdis/jix363
复制
发表时间：
2017-09-15
影响因子：
6.4
通讯作者：
Rochford, Rosemary
中科院分区：
文献类型：
--
作者：
Coleman, Carrie B.;Daud, Ibrahim I.;Rochford, Rosemary
背景EB病毒（EBV）的2种毒株，EBV 1型（EBV-1）和EBV-2，潜伏基因不同，表明它们使用不同的机制来建立潜伏期。我们以前报道过EBV-2在体外感染T细胞。在这项研究中，我们测试了EBV-2在体内感染T细胞的可能性。从EBV-1或EBV-2阳性的儿童及其母亲中分离纯化的T细胞组分用于EBV的存在和EBV类型的检查。我们在12个月龄时从EBV-2感染儿童获得的所有T细胞样本中检测到EBV-2，一些儿童在24个月龄时保留EBV-2阳性T细胞，这表明EBV-2持续存在于T细胞中。我们无法在母亲的T细胞样本中检测到EBV-2，但可以在其母乳和唾液样本中检测到EBV-2。这些数据表明，EBV-2使用T细胞作为额外的潜伏期储存库，但随着时间的推移，感染T细胞的频率可能会下降到可检测的水平以下。或者，EBV-2可以在T细胞区室中建立长期的瞬时感染。总的来说，这些新的发现表明EBV-2在体内感染T细胞，并表明EBV-2可能使用T细胞区室来建立潜伏期。
Background. The 2 strains of Epstein-Barr virus (EBV), EBV type 1 (EBV-1) and EBV-2, differ in latency genes, suggesting that they use distinct mechanisms to establish latency. We previously reported that EBV-2 infects T cells in vitro. In this study, we tested the possibility that EBV-2 infects T cells in vivo.Methods. Purified T-cell fractions isolated from children positive for EBV-1 or EBV-2 and their mothers were examined for the presence of EBV and for EBV type.Results. We detected EBV-2 in all T-cell samples obtained from EBV-2-infected children at 12 months of age, with some children retaining EBV-2-positive T cells through 24 months of age, suggesting that EBV-2 persists in T cells. We were unable to detect EBV-2 in T-cell samples from mothers but could detect EBV-2 in samples of their breast milk and saliva.Conclusions. These data suggest that EBV-2 uses T cells as an additional latency reservoir but that, over time, the frequency of infected T cells may drop below detectable levels. Alternatively, EBV-2 may establish a prolonged transient infection in the T-cell compartment. Collectively, these novel findings demonstrate that EBV-2 infects T cells in vivo and suggest EBV-2 may use the T-cell compartment to establish latency.