Long-term high-concentrate diet feeding induces apoptosis of rumen epithelial cells and inflammation of rumen epithelium in dairy cows
Long-term high-concentrate diet feeding induces apoptosis of rumen epithelial cells and inflammation of rumen epithelium in dairy cows
复制标题
长期高浓度日粮饲喂诱发奶牛瘤胃上皮细胞凋亡及瘤胃上皮炎症
DOI：
10.1080/10495398.2020.1806073
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发表时间：
2020-08-14
影响因子：
3.7
通讯作者：
Shen, Xiangzhen
中科院分区：
文献类型：
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作者：
Dai, Hongyu;Ma, NaNa;Shen, Xiangzhen
摘要本研究旨在探讨长期饲喂高精料日粮对奶牛瘤胃上皮组织的影响。试验选取12头泌乳中期产奶牛，随机分为高精料组（HC，精料：粗料= 6:4）和低精料组（LC，精料：粗料= 4:6），饲喂20周。与LC组相比，HC组瘤胃内脂多糖（LPS）水平显著升高，pH值显著降低。与LC组相比，HC组RE中白细胞介素-6 （IL-6）、白细胞介素-8 （IL-8）、C-C基序趋化因子配体5 （CCL5）、caspase-3、caspase-8、caspase-9 mRNA丰度升高。HC奶牛RE中磷酸化NF-κB p65、IL-6和肿瘤坏死因子α (TNF-α)的蛋白丰度高于LC奶牛。HC组促凋亡反应相关蛋白（细胞色素c、BAX、caspase-3）丰度高于LC组，而抗凋亡因子蛋白（Bcl-2）丰度低于LC组。因此，本研究表明，长期饲喂高精料日粮可上调瘤胃液LPS水平，诱导瘤胃上皮细胞的促炎反应和凋亡。
Abstract The aim of the present study was to evaluate how long-term high-concentrate diet feeding affected rumen epithelium (RE) of dairy cows. So, 12 mid-lactating multiparous cows were divided into two groups randomly fed either with high-concentrate diet (HC, concentrate: forage = 6: 4) or low-concentrate diet (LC, concentrate: forage = 4:6) for 20 weeks. Remarkable upregulation of lipopolysaccharide (LPS) level and depress of pH in rumen fluid were induced by HC compared with LC group. mRNA abundance of interleukin-6 (IL-6), interleukin-8 (IL-8), C-C motif chemokine ligand 5 (CCL5), caspase-3, caspase-8, and caspase-9 were elevated in RE of HC group compared with LC group. Greater protein abundance of phosphorylated NF-κB p65, IL-6, and tumor necrosis factor α (TNF-α) was observed in RE of cows fed HC than that fed LC. Abundance of protein related to proapoptotic response (cytochrome c, BAX and caspase-3) in HC group was greater than that in LC group, while the abundance of anti-apoptotic factor protein (Bcl-2) was lower in HC group than LC group. Therefore, the present study demonstrated that long-term high-concentrate diet feeding upregulated LPS level in rumen fluid and induced the proinflammatory response in the rumen epithelium and apoptosis of rumen epithelial cells.