Nuclear Lamin is required for Winged Eye-mediated transdetermination of Drosophila imaginal disc
Nuclear Lamin is required for Winged Eye-mediated transdetermination of Drosophila imaginal disc
复制标题
核纤层蛋白是翼眼介导的果蝇成虫盘转决定所必需的
DOI：
10.1111/gtc.12608
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发表时间：
2018
期刊：
影响因子：
2.1
通讯作者：
Kurata Shoichiro
中科院分区：
文献类型：
--
作者：
Masuko Keita;Furuhashi Hirofumi;Komaba Kanae;Numao Eriko;Nakajima Rumi;Fuse Naoyuki;Kurata Shoichiro
果蝇生殖盘在应激条件下经常改变其细胞命运，这种现象称为transdetermination（TD），长期以来一直是研究再生过程中细胞命运可塑性的有用模型。我们先前鉴定了一种染色质相关蛋白质，翼眼（Wge），其在眼成虫盘中过度表达时诱导眼到翼TD。然而，Wge介导的TD的分子机制仍然不清楚。在这里，我们分析了Wge相互作用蛋白，发现几种异染色质相关蛋白，包括核纤层蛋白Lamin（Lam），与培养细胞中的Wge蛋白相关。击倒实验表明，Lam确实是Wge介导的眼对翼TD所必需的。此外，Wge过表达改变了细胞核内基因组DNA的空间组织。因此，我们认为，Wge与Lam相互作用，将一些基因组区域与核周边连接，并调节成虫盘TD中的染色质动力学。
Drosophilaimaginal discs often change their cell fate under stress conditions, and this phenomenon, called transdetermination (TD), has long been a useful model for studying cell fate plasticity during regeneration. We previously identified a chromatin‐associated protein, Winged Eye (Wge), which induces eye‐to‐wing TD upon its over‐expression in eye imaginal discs. However, the molecular mechanism of Wge‐mediated TD remains obscure. Here, we analyzed Wge‐interacting proteins and found that several heterochromatin‐related proteins, including a nuclear lamina protein, Lamin (Lam), were associated with Wge protein in cultured cells. Knockdown experiments revealed that Lam is indeed required for Wge‐mediated eye‐to‐wing TD. Moreover, Wge over‐expression altered the spatial organization of genomic DNA inside the cell nuclei. Accordingly, we suggest that Wge interacts with Lam to link some genomic regions with the nuclear periphery and regulates chromatin dynamics in imaginal disc TD.