Molecular cloning of a novel 97-kd Golgi complex autoantigen associated with Sjogren's syndrome
Molecular cloning of a novel 97-kd Golgi complex autoantigen associated with Sjogren's syndrome
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DOI：
10.1002/art.1780400920
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发表时间：
1997-09-01
影响因子：
--
通讯作者：
Fritzler, MJ
中科院分区：
文献类型：
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作者：
Griffith, KJ;Chan, EKL;Fritzler, MJ
目的：鉴定干燥综合征结合的高尔基复合体自身抗原。方法：以1例继发性干燥综合征患者血清和抗高尔基体抗体为探针，从HeLa cDNA文库中克隆出一个3.7kb的cDNA片段，编码56 kd的重组蛋白。免疫兔血清与golgin-97蛋白的分子量为97 kd，表明该克隆含有部分cDNA。通过快速扩增cDNA末端，获得5'上游序列，全长cDNA含有4，860个碱基对，编码分子量为88 kd的蛋白。在60例已知有抗Golgi自身抗体的血清中，有12例（20%）检测到Golgin-97抗体，其中大多数（8/12，75%）来自继发性SS患者。结论Golgin-97是一种独特的Golgi复合体抗原，可能是SS自身抗体的靶点。
Objective, To identify a Golgi complex autoantigen bound by Sjogren's syndromeMethods, Serum from a patient with secondary SS and anti-Golgi antibodies was used as a probe to isolate a complementary DNA (cDNA) insert from a HeLa cDNA library.Results, A 3.7-kb cDNA encoding a 56-kd recombinant protein was immunoprecipitated by the human anti-Golgi serum and immune rabbit serum. Western blot analysis showed that the immune rabbit sera recognized a protein of 97 kd (golgin-97), suggesting that the isolated clone contained a partial cDNA, The 5' upstream sequence was obtained by rapid amplification of the cDNA ends, The complete cDNA contained 4,860 basepairs, encoding a protein with a calculated M-r of 88 kd. Antibodies to golgin-97 were found in 12 (20%) of 60 sera known to have anti-Golgi autoantibodies, and the majority of these sera (8 of 12, or 75%) were from patients who had secondary SS,Conclusion, Golgin-97 is a unique Golgi complex antigen that appears to be a target of SS autoantibodies.