Expression and release of CD27 in human B-cell malignancies.
Expression and release of CD27 in human B-cell malignancies.
复制标题
CD27 在人类 B 细胞恶性肿瘤中的表达和释放。
作者：
M. Van Oers;S. Pals;L. Evers;C. van der Schoot;G. Koopman;J. Bonfrer;RQ Hintzen;A. Von Dem Borne;R. van Lier
CD27是一种跨膜二硫键连接的55kD同源二聚体，属于神经生长因子受体家族，是一组参与淋巴细胞分化和选择的同源分子。它表达在成熟的胸腺细胞、外周血T细胞和B细胞亚群上。我们研究了CD27在恶性B细胞上的表达，这些B细胞代表了生理抗原非依赖性和非依赖性B细胞发育的广泛阶段。正常淋巴组织中CD27+B细胞仅见于外周血(29.8%+/-10.8%，n=13)和生发中心。除PRO-B和大多数PRE-Pre-B急性淋巴细胞白血病和骨髓瘤外，几乎所有未成熟和成熟的恶性B细胞均检测到不同强度的CD27表达。此外，使用夹心酶联免疫吸附试验，我们可以显示在B细胞恶性肿瘤患者的血清中有时存在非常高的(高达6000U/毫升；正常值和190U/毫升)的可溶性28-32kD形式的CD27(SCD27)。慢性淋巴细胞白血病和低度恶性非霍奇金淋巴瘤患者的sCD27水平最高。最重要的是，在横向和纵向研究中，我们发现血清中sCD27水平与肿瘤负荷之间存在很强的相关性，表明sCD27可以作为急、慢性B细胞恶性肿瘤患者的疾病标志物。
CD27, a transmembrane disulfide-linked 55-kD homodimer, belongs to the nerve growth factor-receptor family, a group of homologous molecules involved in lymphocyte differentiation and selection. It is expressed on mature thymocytes, peripheral blood T cells, and a subpopulation of B cells. We investigated the expression of CD27 on malignant B cells representative for a broad range of stages in physiologic antigen-independent and -dependent B-cell development. In normal lymphoid tissue CD27+ B cells were only found in the peripheral blood (29.8% +/- 10.8%, n = 13) and in germinal centers. With the exception of pro-B and the majority of pre-pre-B acute lymphocytic leukemias and of myelomas, CD27 expression of variable intensity was detected on almost all immature and mature malignant B cells tested. Moreover, using a sandwich enzyme-linked immunosorbent assay we could show the presence of sometimes very high (up to 6,000 U/mL; normal values < 190 U/mL) amounts of the soluble 28- to 32-kD form of CD27 (sCD27) in the sera of patients with B-cell malignancies. The highest levels of sCD27 were observed in patients with chronic lymphocytic leukemia and low-grade non-Hodgkin's lymphomas. Most importantly, both in transversal and longitudinal studies, we found a strong correlation between sCD27 levels in the serum and tumor load, indicating that sCD27 can be used as a disease-marker in patients with acute and chronic B-cell malignancies.
CD27 抗原参与 IL-2 激活的人类自然杀伤细胞的调节。
DOI：
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发表时间：
1992
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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作者：
Sugita,K;Robertson,MJ;Torimoto,Y;Ritz,J;Schlossman,SF;Morimoto,C
通讯作者：
Morimoto,C