Glucose oxidase polyion complex-bilayer membrane for elimination of electroactive interferents in amperometric glucose sensor
Glucose oxidase polyion complex-bilayer membrane for elimination of electroactive interferents in amperometric glucose sensor
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DOI：
10.1016/s0003-2670(98)00003-8
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发表时间：
1998-05-25
影响因子：
6.2
通讯作者：
Yabuki, S
中科院分区：
文献类型：
--
作者：
Mizutani, F;Sato, Y;Yabuki, S
将葡萄糖氧化酶（GOx）固定在聚离子复合膜上，制备了葡萄糖电流型传感电极。首先，通过将金电极浸入含有3-巯基丙酸（MPA）的乙醇溶液中，在金电极表面形成单层3-巯基丙酸（MPA）。将聚-L-赖氨酸和聚-4-苯乙烯磺酸盐的水溶液依次置于电极表面上并使其干燥。然后通过加入戊二醛溶液交联酶，在聚-L-赖氨酸/聚-4-苯乙烯磺酸盐复合物层上形成GOx层。聚离子复合物层能有效地消除电化学干扰物，如L-抗坏血酸、尿酸和对乙酰氨基酚。而通过GOx催化的反应产生的过氧化氢迅速渗透通过该层。这导致了快速反应（100%的反应，
An amperometric glucose-sensing electrode was prepared by immobilizing glucose oxidase (GOx) on a polyion complex membrane. First, a monolayer of 3-mercaptopropionic acid (MPA) was made on the surface of a gold electrode by immersing it in an ethanol solution containing MPA. Aqueous solutions of poly-L-lysine and poly-4-styrenesulfonate were successively placed on the electrode surface and allowed to dry. A GOx layer was then formed on the poly-L-lysine/poly-4-styrenesulfonate-complex layer by crosslinking the enzyme by the addition of a glutaraldehyde solution. The polyion complex layer was effective for eliminating electrochemical interferents such as L-ascorbic acid, uric acid and acetaminophen. whereas the hydrogen peroxide produced through the GOx-catalyzed reaction permeated rapidly through the layer. This resulted in a rapid response (100% response in