Chrysanthemum flower shape modification by suppression of chrysanthemum-AGAMOUS gene
Chrysanthemum flower shape modification by suppression of chrysanthemum-AGAMOUS gene
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DOI：
10.5511/plantbiotechnology.25.55
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发表时间：
2008-01-01
影响因子：
1.6
通讯作者：
Murai, Koji
中科院分区：
文献类型：
--
作者：
Aida, Ryutaro;Komano, Masayasu;Murai, Koji
创造新的花形是观赏植物育种的主要目标。已知花结构的ABC模型适用于广泛的植物。抑制C基因会产生重瓣花表型，失去花的确定性。摘要通过抑制可能为C基因的CAG基因，对菊花（chrysanthemum morifolium）的花形进行了修饰。我们获得了103株转基因植株；然而，只有一株（951-2）的花形发生了变化。951-2系每个射线小花的雌蕊都转化为几个花冠样组织（次生花冠）和一个雌蕊样组织。Southern blot分析显示该转基因基因多拷贝整合到951-2基因组中。与野生型相比，951-2系CAG mRNA的表达量减少。在射线小花上，花冠正面的细胞形状在原花冠和次生花冠之间几乎相同。柱头在放射状小花和盘状小花上发育不良，结构平实。我们在951-2中观察到异常宽的细丝。异常丝表面细胞结构转变为花冠状细胞。在这项研究中，我们证明了CAG基因的抑制使雄蕊和雌蕊转化为花冠样组织。
Creation of new flower shapes is a major breeding target for ornamental plants. The ABC model on the flower structure is known to be applicable to a broad range of plants. Suppression of the C gene would produce a double flower phenotype with loss of floral determinacy. The flower shape of chrysanthemum (Chrysanthemum morifolium) was modified by suppressing the chrysanthemum-AGAMOUS (CAG) gene, which might be a C gene, with an antisense transgene. We obtained 103 transgenic plants; however, only a single line (951-2) showed modified flower shape. The pistil of each ray floret in line 951-2 was transformed to several corolla-like tissues (secondary corolla) and a pistil-like tissue. Southern blot analysis showed multiple-copy integration of the transgene into the 951-2 genome. The amount of CAG mRNA was reduced in line 951-2 compared to the wild-type. On the ray florets, the cell shapes of the adaxial surfaces of the corolla seemed to be almost the same between the proper corolla and the secondary corolla. The stigma was poorly developed and plain structured both on the ray and disk florets. We observed abnormally wide filaments in 951-2. The structure of the surface cells of the abnormal filaments transformed to corolla-like cells. In this study, we demonstrate that suppression of the CAG gene converted the stamen and pistil into corolla-like tissues.